1. Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student Oral presentation CREATE Gjøvik

2. There will be: Short introduction to fluorescence Few applications –Something about bioanalytics –Coloring or whitening merchandise –Arts Measurements and data analysis –by using two monochromator –method Fluorescence : One phenomenon, many applications / Jussi Kinnunen 2

3. What is fluorescence?

4. For me it is fancy colours Fluorescence : One phenomenon, many applications / Jussi Kinnunen 4

5. Light interaction with matter When light comes Absorption –Time about 10 -15 s Scattering –Time about 0 s When light goes Fluorescence emission –Lifetime about 10 -8 (10 -10 ) s –Lower energy photons than the one(s) absorbed Phosphorescence –Lifetime >10 -8 s –Lower energy photons than the one(s) absorbed Scattering –Energy if the photon is close to the incident one(s) Fluorescence : One phenomenon, many applications / Jussi Kinnunen 5

6. Applications: Bioanalytics

7. Fast detection of small quantities of anything Idea: –Choose a receptor for the target –Attach label (reporter) to the receptor –Illuminate and see Where is your target –e.g. cancer cells Was there any of your target –e.g. DNA sequencing Fluorescence : One phenomenon, many applications / Jussi Kinnunen 7

8. Applications: Industry

9. Making things white Fluorescence : One phenomenon, many applications / Jussi Kinnunen 9

10. Applications: Art

11. Masterpiece (Miera, 1950’s), white light and UV Photo courtesy of Nick Padalino Fluorescence : One phenomenon, many applications / Jussi Kinnunen 11

12. Color measurements of fluorescence

13. Two monochromator method Fluorescence : One phenomenon, many applications / Jussi Kinnunen 13

14. Measured data Donaldson matrix is calculated from the measured data The reflectance –On diagonal The fluorescence –Off diagonal Fluorescence : One phenomenon, many applications / Jussi Kinnunen 14

15. Simulations

16. Simulation with the scientific brown Fluorescence : One phenomenon, many applications / Jussi Kinnunen 16

17. Simulation with the scientific brown Fluorescence : One phenomenon, many applications / Jussi Kinnunen 17

18. Lights Simulations under –Tungsten light (green) –Daylight simulator (blue) –White led (red) –Fluorescent light (cyan) Fluorescence : One phenomenon, many applications / Jussi Kinnunen 18

19. Under “smooth” light sources Halogen lightDaylight simulator Fluorescence : One phenomenon, many applications / Jussi Kinnunen 19 reflectance fluorescence total The black curve is measured under polycromatic light

20. Under “peaky” light sources Led lightFluorescent light Fluorescence : One phenomenon, many applications / Jussi Kinnunen 20 reflectance fluorescence total The black curve is measured under polycromatic light

21. Thank you. www.uef.fi