1. Candace Parchen (BS Cell Molec. Biol. 2004) will give the Biology seminar next Wednesday 2/10 (4pm in BI 234). Title: The Effect of Phosphodiesterase 5 (PDE5) inhibitors on Dystrophic Hearts Annual T-shirt contest is underway. Strut your geeky stuff!!!!!

3. Figure 19-50 Molecular formula of the phosphatidylinositides. Page 707 Chapter 19 Section 4 Site of PLC cleavage Usually stearic acid and arachidonic acid What are they?

4. Figure 19-51Role of PIP 2 in intracellular signaling. Page 708 How does Ca +2 release occur? PHOSPHONOSITIDE CASCADE! Epinephrine Glucagon, etc Dag remains in membrane 2 nd messenger

5. Figure 19-52A phospholipase is named according to the bond that it cleaves on a glycerophospholipid. Page 709

6. Membrane Transport Ch 20

9. Figure 20.2 Movement across membrane varies linearly with concentration flux

11. “non-mediated” transport

12. Figure 20.4 Simple facilitated diffusion Transport protein

13. Figure 20.6 Two types of ionophores

14. Figure 20-8 Valinomycin (K + complex) Carries 10 4 K + /sec!!!!!!! 10,000 fold discrimination against Na + ! HOW???

15. Figure 20-8bValinomycin X- ray structures. (b) Uncomplexed valinomycin. Page 731

16. Figure 20-9aMonensin. (a) The structural formula with the six oxygen atoms that octahedrally complex Na + indicated in red. Page 731

17. Figure 20-9bMonensin. (b) The X-ray structure of the Na + complex (H atoms not shown). Page 731

18. Figure 20-9 Monensin (Na + complex)

19. Figure 20-10Gramicidin A. This polypeptide consists of 15 alternating D- and L-amino acid residues and is blocked at both its N- and C-termini. Page 732

20. Figure 20-11aNMR structure of gramicidin A embedded in a dimyristoyl phosphatidylcholine bilayer. (a) View from within the bilayer along the homodimeric helix’s twofold axis. Page 732

21. Figure 20-11bNMR structure of gramicidin A embedded in a phospholipid bilayer rotated 90° about the horizontal axis relative to Part a. Page 732

22. Gramicidin A See Figure 20.11  -hemolysin (Staph. aureus) “IONOPHORES”

24. Table 20-1

25. Figure 20-13Predicted secondary structure and membrane orientation of the glucose transporter. Page 734

26. Figure 20.14

27. Figure 20.15

35. Figure 20-24a X-Ray structure of the Ca 2+ – ATPase from rabbit muscle sarcoplasmic reticulum. (a) A tube-and-arrow diagram. Page 743

36. Figure 20-24b X-Ray structure of the Ca 2+ – ATPase from rabbit muscle sarcoplasmic reticulum. (b) A schematic diagram of the structure viewed similarly to Part a. Page 743

37. Figure 20-25Transport of glucose by the PEP-dependent phosphotransferase system (PTS). Page 745

38. Figure 20-27abGlucose transport in the intestinal epithelium. Page 747

39. Figure 20-27cGlucose transport in the intestinal epithelium. Page 747

40. Figure 20-28The Na + –glucose symport system represented as a Random Bi Bi kinetic mechanism. Page 748

41. Figure 20-30Conformational mechanism of the ATP–ADP translocator. Page 750

43. Figure 20.18

44. Table 20-3

45. Figure 20.21

47. Figure 20.27

49. “Alfonse, Biochemistry makes my head hurt!!” \