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1 Energy –capacity to do work or cause change Endergonic reactions – consume energy Exergonic reactions – release energy
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2 Energy Transfer reactions catalyzed by enzymes 1.Oxidation-reduction reactions – transfer of electrons 2.Phosphotransferases – transfer phosphate groups, involved in energy transfer
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3 Redox reactions always occur in pairs There is an electron donor and electron acceptor which constitute a redox pair The process salvages electrons & their energy. released energy can be captured to phosphorylate ADP or another compound
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4 Electron carriers resemble shuttles that are loaded and unloaded with electrons and hydrogen most carriers are coenzymes, NAD, FAD, NADP, coenzyme A & compounds of the respiratory chain
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5 Electron carriers
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6 ATP 3 part molecule consisting of –adenine – a nitrogenous base –ribose – a 5-carbon sugar –3 phosphate groups Removal of the terminal phosphate releases energy
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7 ATP
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8 Formation of ATP 1.substrate-level phosphorylation 2.oxidative phosphorylation 3.photophosphorylation
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9 substrate-level phosphorylation
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10 Catabolism of glucose 1.Glycolysis 2.Tricarboxylic acid cycle, Kreb’s cycle 3.Respiratory chain, electron transport
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11 Metabolic strategies Pathways involved Final e- acceptor ATP yield Aerobic respiration Glycolysis, TCA, ET O2O2 38 Anaerobic respiration Glycolysis, TCA, ET NO 3 -, So 4 -2, CO 3 -3 variable FermentationGlycolysisOrganic molecules 2
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12 Overview of aerobic respiration
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13 Overview of aerobic respiration Glycolysis – glucose (6C) is oxidized and split into 2 molecules of pyruvic acid (3C) TCA – processes pyruvic acid and generates 3 CO 2 molecules Electron transport chain – accepts electrons NADH & FADH, generates energy through sequential redox reactions called oxidative phosphorylation
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14 Glycolysis
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15 TCA cycle
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16 Electron transport system
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17 Chemiosmosis
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18 Fermentation Incomplete oxidation of glucose or other carbohydrates in the absence of oxygen Uses organic compounds as terminal electron acceptors Yields a small amount of ATP Production of ethyl alcohol by yeasts acting on glucose Formation of acid, gas & other products by the action of various bacteria on pyruvic acid
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19 Fermentation
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20 Products of fermentation
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21 Many pathways of metabolism are bi-directional or amphibolic Metabolites can serve as building blocks or sources of energy –Pyruvic acid can be converted into amino acids through amination –Amino acids can be converted into energy sources through deamination –Glyceraldehyde-3-phosphate can be converted into precursors for amino acids, carbohydrates and fats
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