1. Bacterial Physiology (Micr430) Lecture 18 Bacterial Pathogenesis (Based on other textbooks such as Madigan’s)

2. Microbe-Host Relationships If a microbe is not lost or shed from the host after entry into the host (animal or plant), there are a few types of relationships that could develop: Commensalism: a coexistance that is harmless to the microbe and host; Symbiosis: a mutual benefit to both the microbe and host; Parasitism: a benefit to the microbe at the expense of the host.

3. Definitions Pathogen: An organism, usually a microorganism, that causes disease to another organism (animal or plant) Infection: Growth of an organism within a host Disease: Injury to the host that impairs host function Infectious Diseases: Diseases caused by the growth of pathogenic microorganisms in the host body

4. Overview of Human-Microbial Interactions Animal bodies are favorable environments for the growth of microorganisms, most of which do no harm. The ability of a pathogen to cause disease is called pathogenicity. An opportunistic pathogen causes disease only in the absence of normal host resistance.

5. Mucous Membranes Pathogen growth on the surface of a host, often on the mucous membranes, may result in infection and disease. Mucous membranes are often coated with a protective layer of viscous soluble glycoproteins called mucus. The ability of a microorganism to cause or prevent disease is influenced by complex host-parasite interactions.

7. Normal Microbial Flora of Skin The skin is a generally dry, acidic environment that does not support the growth of most microorganisms. However, moist areas, especially around sweat glands, are colonized by gram- positive Bacteria and other members of the skin normal flora. Environmental and host factors influence the quantity and quality of the normal skin microflora.

8. Normal Microbial Flora of Skin The normal flora of the skin consists of transient or resident populations of microorganisms. The skin is continually being inoculated with transient microorganisms, virtually all of which are unable to multiply and usually die. Resident microorganism are able to multiple, not merely survive, on the skin.

9. Normal Flora of the Oral Cavity Bacteria can grow on tooth surfaces in thick layers called dental plaque. Plaque microorganisms produce adherent substances. Acid produced by microorganisms in plaque damages tooth surfaces, and dental caries result. A variety of microorganisms contribute to caries and periodontal disease.

10. Normal Flora of the GI Tract The stomach is very acidic and is a barrier to most microbial growth. The intestinal tract (Figure 21.8) is slightly acidic to neutral and supports a diverse population of microorganisms in a variety of nutritional and environmental conditions.

13. Normal Flora of Respiratory Tract In the upper respiratory tract (nasopharynx, oral cavity, and throat), microorganisms live in areas bathed with the secretions of the mucous membranes. The normal lower respiratory tract (trachea, bronchi, and lungs) has no resident microflora, despite the large numbers of organisms potentially able to reach this region during breathing.

14. Normal Flora of Respiratory Tract The presence of a population of normal nonpathogenic microorganisms in the respiratory tract is essential for normal organ function and often prevents the colonization of pathogens.

15. Harmful Microbial Interactions with Humans

16. Definitions Pathogenicity: the ability to cause disease in a host The degree of pathogenicity is called virulence Attenuation is loss of virulence.

17. Entry of the Pathogen into the Host Pathogens gain access to host tissues by adherence to mucosal surfaces through interactions between pathogen and host macromolecules. Table 21.3 gives major adherence factors used to facilitate attachment of microbial pathogens to host tissues. Pathogen invasion starts at the site of adherence and may spread throughout the host via the circulatory systems.

19. Colonization and Growth A pathogen must gain access to nutrients and appropriate growth conditions before colonization and growth in substantial numbers in host tissue can occur. Organisms may grow locally at the site of invasion or may spread through the body. If extensive bacterial growth in tissues occurs, some of the organisms are usually shed into the bloodstream in large numbers, a condition called bacteremia.

20. Process of Microbial Infection that Causes a Disease

21. Virulence Virulence is determined by invasiveness, toxicity, and other factors produced by a pathogen (Figure 21.16). Various pathogens produce proteins that damage the host cytoplasmic membrane, causing cell lysis and death. Virulence is measured by the LD 50 (the dose of an agent that kills 50% of the animals in a test group) or ID 50

23. Virulence Factors Pathogens produce a variety of enzymes that enhance virulence by breaking down or altering host tissue to provide access and nutrients. Still other pathogen-produced virulence factors provide protection to the pathogen by interfering with normal host defense mechanisms. These factors enhance colonization and growth of the pathogen.

24. Exotoxins The most potent biological toxins are the exotoxins produced by microorganisms. Each exotoxin affects specific host cells, causing specific impairment of a major host cell function. Mechanism of cholera toxin will be discussed.

25. Mode of Action For Cholera Toxin

26. Mode of Action For Cholera Toxin