1. Simple ELISA protocol (For screening monoclonal antibodies) 1. Coat antigen onto microplate 2. Allow protein adsorption and block unoccupied sites with neutral protein 3. Add antibody solution (hybridoma supernatant) into each well 4. Add HRP or AP conjugated secondary antibody into each well and develop colorimetric reaction with appropriate substrate 5. Read absorbance in spectrophotometer with appropriate filter and quantitate relative antigen levels

2. Antibody-based Protein Array 1. Incubate soluble sample on plate or membrane-immobilized antibody array 2. Add a mix of labeled soluble antibody against the same set of antigens 3. Incubate with developing system and quantitate signal Full set of data 123456123456 123456123456 123456123456

3. Chromatin Immunoprecipitation Assay (ChIP) 1. Crosslink Protein-DNA complexes in situ 2. Isolate nuclei and fragment DNA (sonication or digestion) 3. Immunoprecipitate with antibody against target nuclear protein and reverse crosslinks 4a. Identify protein components of isolated complexes 4b. Identify DNA sequence by PCR, cloning and sequencing

4. To order ScienceSlides for PowerPoint please go to www.VisiScience.com ScienceSlides