1. Nucleic Acids 101

2. Last week’s grand challenge: Reading and interpreting genetic information

7. DNA Repair: DNA polymerase 10 -7 Mismatch repair 10 -9

8. Genomics

9. Restriction Nucleases

10. Ligation

11. Grand challenge: Every disease requires a tremendous amount of interdisciplinary effort to understand and fight it. Can this be streamlined?

16. The frequency of crossing-over is ~proportional to the physical distance between them. Find DNA markers close to and flanking the gene of interest

17. Positional Cloning A chromosome has a single DNA molecule running the length of the chromosome Map the gene for the trait in question in that DNA molecule; identify close flanking markers “Walk” between the flanking markers Identify the gene in question between the markers

18. How do you map a gene? Collect DNA samples from family members in a pedigree Seek linkage to a polymorphic DNA marker Take advantage of the Human Genome Project to identify the chromosomal region Narrow down the location in the overlapping clones

19. What are “DNA markers”? RFLP--Restriction Fragment Length Polyhmorphism SNP--Single Nucleotide Polymorphisms

20. Mutation destroys a restriction site, causing a RFLP (This is also a SNP--Single Nucleotide Polymorphism)

21. RFLP

22. STRP or VNTR

23. Pedigree of Huntington Disease

24. SNP’s: Single Nucleotide Polymorphisms

25. NEXT CLASS Central Dogma