1. Experimental Annotations in ROC Russ Altman, Alain Laederach Based on our experiences with RiboWeb and our participation in a Program Project Grant

2. Chalk Talk

3. Structural Information PDB –Evidence for Structural Motifs Base Pairs, Long range interactions etc. Experiments –Many Biochemists getting more or less accurate structural evidence as well DMS Footprinting, Hydroxyl Radical Footprinting Tethered BABE probes, FRET, NMR, EPR –Even computational folks predict structure Phylogenetic co-variance analysis M-Fold

4. A possible hierarchy

5. Why is this useful? We can populate instances from PDB We can also populate from experiments –Biochemical –Computational For structure prediction: –Make PDB from ensemble of biochemical data