1. Connective Tissue Slide and Image Orientations Sun-Kee Kim, Ph.D. (kimsk@umich.edu)kimsk@umich.edu

2. Loose (areolar) connective tissue Cells > fibers and ground substance #29 #30

3. #29 Small Intestine

4. Collagen fibers and fibroblasts

5. #29 – Intestinal Villi

6. Lamina propria (Loose conn. tissue)

7. #20 or #40 Trachea

8. Plasma cells

9. Eccentrically located nucleus Cartwheel appearance of nucleus Basophilic cytoplasm Egg-shaped cell with clear outline

10. #40 Respiratory epithelium

11. #26 lymph node

12. Macrophages Kidney bean shaped nucleus (indented) Eosinophilic cytoplasm with bubbly appearance Ingested materials in the cytoplasm Fuzzy cell border (outline)

13. #152 Pharynx - fat cells

14. Slide 160 PAS & Azure Blue: Mast cells

15. Mast cell

16. #27 Lymph node stained with H & E – Reticular connective tissue

17. #28 Lymph node stained with silver – Reticular connective tissue

18. Dense regular and irregular connective tissue Cells < fibers and ground substance #106 or 112 dermis tendon

19. #33 Dermis of skin (aldehyde fuchsin stain) Dense irregular connective tissue with elastic fibers

20. #250 Vagina ( Dense irregular CT) H & E Trichrome

21. #112 (or #106) Musculo-tendonous junction

22. #36 Aorta (elastin sheets)