1. System Biology Team Project Chemicals- Tryptophan 組員： 林瑋耿, 吳東耘, 許博凱, 彭嘉冠

2. System Biology Tryptophan Molecular formula –C 11 H 12 N 2 O 2 Molecular weight –204.23 Isoelectric point –pH 5.89 CAS number –73-22-3 Functional group : indole

3. System Biology Where to get it ? Tryptophan was available in health food –Banana, milk, fish, and etc. …

4. System Biology What are its usage ? Tryptophan is effective sleep aid. It shows considerable promise as an antidepressant. Other promising indications include reduce some emotional disorders. –Impulsive, violent, and etc. disorders

5. System Biology Cont. 人類必需胺基酸之一種 ( 無法自行合成 ) 合成 serotonin 的原料, 是神經傳導物質之一種, 能 抑制食慾產生飽足感 合成 melatonin 的原料, 能幫助睡眠, 安定心情, 增 強免疫力, 常被用來調整時差 Serotonin 和 melatonin 的量會隨著日夜而波動, 推 測與生理時鐘的調節有關 維生素 B3 ( 菸鹼酸, niacin) 的原料, 作為輔脢 (coenzyme) 幫助代謝作用進行 植物生長激素 IAA (auxin) 的原料

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8. NAD + & NADH 2e - + 2H +

9. System Biology Tryptophan Biosynthesis

10. System Biology Inhibited by L-tyrosine Inhibited by L-tryptophan Inhibited by L-phenyalanine 1 2 5 3 4 6 7 1 - Phospho-2-dehydro-3-deoxyheptonate aldolase [EC:2.5.1.54]2.5.1.54 2- 3-dehydroquinate synthase [EC:4.2.3.4]4.2.3.4 3- 3-dehydroquinate dehydratase (3-dehydroquinase) [EC:4.2.1.10]4.2.1.10 Enolase [EC:4.2.1.11]4.2.1.11 4- shikimate 5-dehydrogenase [EC:1.1.1.25]1.1.1.25 5- shikimate kinase II (SKII) [EC:2.7.1.71]2.7.1.71 6- 3-phosphoshikimate 1-carboxyvinyltransferase [EC:2.5.1.19]2.5.1.19 7- chorismate synthase [EC:4.2.3.5]4.2.3.5

11. System Biology Inhibited by L-tryptophan 8 & 9- anthranilate phosphoribosyltransferase / anthranilate synthase component II [EC:2.4.2.18, 4.1.3.27]2.4.2.184.1.3.27 10 & 11- indole-3-glycerol phosphate synthase (IGPS) / N-(5'-phospho-ribosyl)anthranilate isomerase (PRAI) [EC:4.1.1.48, 5.3.1.24]4.1.1.485.3.1.24 12- tryptophan synthase [EC:4.2.1.20]4.2.1.20 8 9 11 10 12

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13. Tryptophan operon in E.coli trpD + trpE = enzyme 8 & 9 trpC = enzyme 10 & 11 trpA + trpB = enzyme 12 Control the 5 reactions from chorismate to tryptophan !!

14. System Biology Trp operon mechanism

15. System Biology References KEGG: http://www.genome.jp/kegg/http://www.genome.jp/kegg/ EcoCyc: http://www.ecocyc.com/http://www.ecocyc.com/ free encyclopedia: http://en.wikipedia.org/wiki/Main_Page http://en.wikipedia.org/wiki/Main_Page

16. System Biology The End