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Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure. 4.Missing factors in E.coli S-30 extract
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100 75 50 37 25 15 10 S1 S2 S4 S5 S13? S16? S?
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“Specialized” ribosome GFP X W X W Ribosome N N S N AGUGTAU UCACAUA
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Future Steps and Issues 1.Increase translation efficiency. 2.Reconstitute 30S and 50S subunits. 3.Genome organization. 4.Autogenous regulation. 4. RNA processing & modifications.
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