1. Tucson High School Biotechnology Course Spring 2010

2. What have we done so far? psbA DNA pol g23 negative control virus DNA positive control negative control virus DNA positive control negative control virus DNA positive control

3. What samples should we see a band for? psbA DNA pol g23 negative control virus DNA positive control negative control virus DNA positive control negative control virus DNA positive control NONO NO YESYES YES MAYBEMAYBE MAYBE

4. Interpreting your gel psbADNApolg23 Negative controlPositive controlVirusNegative controlPositive controlVirus Negative controlPositive controlVirus

5. What should you write about your gel?

6. MyovirusPodovirus psbA DNA pol g23 XX X X Hypothesis

7. Next… Sequencing Need to Clean Up DNA PCR reaction taq polymerase dNTPsprimers MgCl 2 bufferDNA DNA clean up

8. MinElute pcr Clean-Up pcrproducts Buffer PB MinEluteColumn MinEluteColumn Buffer PE MinEluteColumn Buffer EB KEEP!!

9. Which pcr products will you clean up? psbADNApolg23 Negative controlPositive controlVirusNegative controlPositive controlVirus Negative controlPositive controlVirus