1. 9 Chromosomes, the Cell Cycle, and Cell Division

2. 9 Systems of Cell Reproduction Four events occur before and during cell division:  A signal to reproduce must be received.  Replication of DNA and vital cell components must occur.  DNA must be distributed to the new cells.  The cell membrane or cell wall must separate the two new cells.

3. 9 Systems of Cell Reproduction Prokaryotes divide by fission.  Most prokaryotes have one circular chromosome.  As DNA replicates, each of the two resulting DNA molecules attaches to the plasma membrane.  As the cell grows, new plasma membrane is added between the attachment points, and the DNA molecules are moved apart.  Cytokinesis separates the one cell into two, each with a complete chromosome.

4. Figure 9.2 Prokaryotic Cell Division

5. 9 Systems of Cell Reproduction Eukaryotic cells divide by mitosis or meiosis. Three Steps:  The replication of the DNA within the nucleus  The packaging and segregation of the replicated DNA into two new nuclei (nuclear division)  The division of the cytoplasm (cytokinesis)

6. 9 Interphase and the Control of Cell Division The cell cycle has two phases: mitosis and interphase. A typical eukaryotic cell will spend most of its life in interphase, the period between divisions of the cytoplasm. Some cells lose the capacity to divide altogether and stay in interphase indefinitely, while other cells divide regularly or occasionally. A Lily cell in interphase

7. 9 Interphase and the Control of Cell Division Interphase consists of three subphases:  G1 (Gap 1): period just after mitosis and before the beginning of DNA synthesis.  S (synthesis): the time when the cell’s DNA is replicated.  G2 (Gap 2): the time after S and prior to mitosis.

8. 9 Eukaryotic Chromosomes Chromatin: the basic unit of the eukaryotic chromosome; a gigantic, linear, double-stranded molecule of DNA complexed with many proteins. Chromatids: what each chromosome consists of after the DNA of a chromosome replicates during S phase. Centromere

9. 9 Eukaryotic Chromosomes Chromosomes are wrapped around proteins called histones. These wraps of DNA and histone proteins are called nucleosomes. The core of a nucleosome contains eight histone molecules, two each from four of the histone classes. During mitosis and meiosis, the chromatin becomes even more coiled and condensed.

10. Figure 9.6 DNA Packs into a Mitotic Chromosome

11. 9 Mitosis: Distributing Exact Copies of Genetic Information Centrosomes:  Replicated when the cell enters S phase and DNA is replicated.  Separate from each other during G2→M.  The orientation determines the cell’s plane of division.  Regions where microtubules form. Parent daughter pairs of centrioles before migration.

12. 9 Mitosis: Distributing Exact Copies of Genetic Information Prophase marks the beginning of mitosis.  Chromosomes compact and coil, becoming more dense and visible.  Polar microtubules form between the two centrosomes and make up the developing spindle.  The mitotic spindle serves as a “railroad track” along which chromosomes will move later in mitosis.  Late in prophase, the kinetochores develop in the region around the centromere and are the sites where microtubules attach to the chromatids.

13. Figure 9.8 Mitosis (Part 1)

14. 9 Mitosis: Distributing Exact Copies of Genetic Information During metaphase, the kinetochores arrive at the equatorial plate.  Chromosomes are fully condensed.  Unraveling of the interconnected DNA molecules at the centromere occurs. Cell in metaphase.

15. Figure 9.8 Mitosis (Part 2)

16. 9 Mitosis: Distributing Exact Copies of Genetic Information Anaphase begins when the centromeres separate.  Molecular motors at the kinetochores move the chromosomes toward the poles.  Also, the microtubules are shortened at the poles, and the mitotic centers further separate. Cells in mid and late anaphase.

17. 9 Mitosis: Distributing Exact Copies of Genetic Information Telophase begins when the chromosomes finish moving.  Nuclear envelopes and nucleoli coalesce and re-form. Animal cells divide by a furrowing (a “pinching in” or constriction) of the plasma membrane. Cell in late telophase. Sea urchin egg at cytokinesis.

18. 9 Reproduction: Asexual and Sexual Mitosis by repeated cell cycles can give rise to vast numbers of genetically identical cells. Meiosis results in just four progeny, which usually do not further duplicate. The cells can be genetically different.

19. 9 Reproduction: Asexual and Sexual Sexual reproduction involves meiosis. Two parents each contribute a set of chromosomes in a sex cell or gamete. Gametes fuse to produce a single cell, the zygote. This creates variety among the offspring beyond that attributed to mutations or the environment.

20. 9 Reproduction: Asexual and Sexual In multicellular organisms, somatic cells each contain two sets of chromosomes. One comes from each of the two parents. The members of the pair are called homologous chromosomes and have corresponding but generally not identical genetic information. Eye Color Hair Color Hairline Ear lobe attachment Aa bB Cc dD Homologous Chromosomes

21. Figure 9.12 Fertilization and Meiosis Alternate in Sexual Reproduction (Part 2) * When haploid gametes fuse in fertilization, they create the diploid zygote (2n). * Haploid cells contain just one homolog of each pair (n).

22. 9 Reproduction: Asexual and Sexual Cells in metaphase can be killed and prepared in a way that spreads the chromosomes around a region on a glass slide. The images of each chromosome can be organized based on size, number, and shape = karyotype.

23. 9 Meiosis: A Pair of Nuclear Divisions Meiosis consists of two nuclear divisions that reduce the number of chromosomes to the haploid number. The DNA is replicated only once. The functions of meiosis:  To reduce the chromosome number from diploid to haploid.  To ensure each gamete gets a complete set of chromosomes.  To promote genetic diversity among products.

24. 9 Meiosis: A Pair of Nuclear Divisions Meiosis I is preceded by an interphase in which DNA is replicated. During prophase I, synapsis occurs: The two homologs are joined together by a complex of proteins. This forms a tetrad, or bivalent, which consists of two homologous chromosomes with two sister chromatids.

25. Figure 9.14 Meiosis (Part 1)

26. 9 Meiosis: A Pair of Nuclear Divisions At a later point, the chromosomes appear to repel each other except at the centromere and at points of attachments which appear x-shaped. These attachment points reflect the exchange of genetic material between homologous chromosomes, a phenomenon called crossing- over. This crossing-over increases genetic variation by reshuffling the genes on the homologs.

27. Figure 9.16 Crossing Over Forms Genetically Diverse Chromosomes

28. 9 Meiosis: A Pair of Nuclear Divisions The homologous chromosomes separate in anaphase I. The individual chromosomes are pulled to the poles, with one homolog of a pair going to one pole and the other homolog going to the opposite pole.

29. Figure 9.14 Meiosis (Part 2)

30. Figure 9.14 Meiosis (Part 3)

31. 9 Meiosis: A Pair of Nuclear Divisions The second meiotic division separates the chromatids. Meiosis II is similar to mitosis but one difference is that DNA does not replicate before meiosis II. The number of chromosomes in the resulting cells is therefore half that found in diploid mitotic cells. In meiosis II, sister chromatids are not identical and there is no crossing-over.

32. Figure 9.14 Meiosis (Part 4)

33. Figure 9.14 Meiosis (Part 5)

34. Figure 9.14 Meiosis (Part 6)

35. 9 Meiosis: A Pair of Nuclear Divisions Meiosis leads to genetic diversity. Synapsis and crossing-over during prophase I mix genetic material of the maternal with that of the paternal homologous chromosomes. Which member of a homologous pair segregates or goes to which daughter cell at anaphase I is a matter of chance. This phenomenon is called independent assortment. Metaphase IAnaphase I

36. 9 Meiotic Errors Nondisjunction occurs when homologous chromosomes fail to separate during anaphase I, or sister chromatids fail to separate during anaphase II. The result is a condition called aneuploidy.

37. Figure 9.18 Nondisjunction Leads to Aneuploidy