Download presentation
Presentation is loading. Please wait.
1
Use of dialysis to separate small and large molecules
2
Gel filtration chromatography
3
Molecular mass determination by gel filtration chromatography
4
Some commonly used gel filtration materials
5
Ion-exchange chromatography with stepwise elution
6
Device for generating a linear concentration gradient
7
Molecular formulas of cellulose-based ion exchangers
8
Some biochemically useful ion exchangers
9
Affinity chromatography
10
Covalent linking of ligand to agarose
11
Derivatization of epoxy-activated agarose
12
Purification of staphylococcal nuclease by affinity chromatography on bisphosphothymidine-linked agarose
13
Experimental arrangement for paper chromatography
14
Two-dimensional paper chromatography
15
Paper electrophoresis
16
Apparatus for slab gel electrophoresis
17
Diagram of a disc electrophoresis apparatus
18
Polymerization of acrylamide and N,N-methylenebisacrylamide to form a cross-linked polyacryl-amide gel
19
SDS-PAGE
20
Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE
21
General formula of the ampholytes used in isoelectric focusing
22
Isoelectric points of several common proteins
23
Purification of rat liver glucokinase
24
END
25
An enzyme-linked immunosorbent assay (ELISA)
26
Solubilities of several proteins in ammonium sulfate solutions
27
Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type
28
Solubility of -lactoglobin as a function of pH at several NaCl concentrations
29
Protein crystals
30
Agarose gel electrophoretogram of a double helical DNA
31
Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs
32
Detection of proteins by immunoblotting
33
Two-dimensional (2D) gel electrophoresis
34
Sedimentation coefficients in Svedbergs (S) for some biological materials
35
A selection of preparative ultracentrifuge rotors
36
Zonal ultracentrifugation
37
Equilibrium density distribution of a CsCl solution in an ultracentrifuge spinning at 39,460 rpm
38
Isopycnic ultracentrifugation
39
Physical constants of some proteins
Similar presentations
© 2024 SlidePlayer.com. Inc.
All rights reserved.