1. Västerhus – information and some results Last edit 080808

2. Seed orchardTestedAvailableNormal order statistics of i:th ranking (= BV) NoNameclones 1234567 412Domsjöänget52422.1801.7761.5421.3701.2321.1141.009 AC1006Y4507AC2064Z3009AC3040AC3033AC4221 410Robertsfors12101.5391.0010.656 Y3014AC3065 18Brån34271.9981.5631.3061.1150.9570.8200.699 AC3056Y3001AC1075AC2047 403Nedansjö40322.0701.6471.4001.2171.0670.9380.824 Y4016Z2081Z4003X4203Z4019 411Domsjöänget50402.1611.7531.5171.3441.2031.0830.977 Y2005Y3012Y2004Z4022Z3029Z4032AC3015 426Holm20161.7661.2850.9900.7630.570 Z3007Y4508Y4103 Selection of clones from six progeny-tests (old orchards)

3. Clone ID Breeding Value Percentage Clone ID Breeding Value Percentage AC10062.27Z40221.33 Y20052.28AC10751.32 Y40162.17Y45081.33 AC30562.09AC30401.23 Y45071.86X42031.22 Z30071.86Z30291.22 Y30121.86AC20471.11 Z20811.66AC30331.11 Y30011.64Z40321.11 AC20641.55Z40191.12 Y30141.55AC42211.02 Y20041.52AC30651.01 Z40031.43Y41031.01 Z30091.42AC30151.01

4. Recommended area for using the seed orchard Västerhus (T10) Latitude for plus tree origin 63°30' Category: Tested Location: Latitude 63,8 N Elevation 15 m Established 1991

5. Range of origins = v = 3 latitudes, but considering pollen, range of halfsibfamilies =v/2 = 1.5 latitude Plust tree origins Seed orchard location

6. Relative performance over plantation sites compared to an optimal seed orchard (=100%)

7. Map. Västerhus + = missing Z3007 = clone ID Parcells (plots) and in plots X for column and Y for row This is actually the only way to find the identity of a particular clone in the orchard! Z3007-82-1-3

8. Västerhus (Anders F 07-10-10)

9. Air picture of Västerhus

10. Västerhus spring 2006. Czech visitors. Photo: Morogushi Västerhus spring 2006

12. Anders and me selected study trees and harvested needles Criteria – 3 ramets per clone Possible to study pollen spreading from rare clones Somewhat spread over the orchard Needles in Xiao-Ru’s lab

13. Cone collection

14. Simple Breeding without Breeding Cycle Seed orchard Forest Group merit selection

15. Västerhus We are anyway genotyping the seed orchard and its offspring There are a few mature (30 year) crosses among seed orchard trees (in old seed orchards, Domsjöänget). Johan Kroon will help us to find some such trees and we will check the possibilities to make phenotypic selection followed by parent determination in a situation where we know the parents. Not many trees. A few days in the summer 2008 to look at the material.

16. Mature seed orchard Domsjöänget, 25 kms from Västerhus. These trees were crossed in the orchard and the progeny planted in field trial. The field trials were evaluated and the best clones placed in Västerhus. Some of the crosses are between Västerhus clones and exist in a 30 year old progeny-test where we collected needles June 2008. When we can evaluate the chance that we can identify the parents

17. Trees at the progeny trial Kvarntorp with crosses at Domsjöänget at needle collection June 2008 Needles are collected by cutting branches down with a pole with a saw (at the centre of the picture) Photo Takeshi Torimaru 0806

18. Plantation with Västerhus seeds

20. Harvesting needles from plantation with Västerhus

21. Half sib forestry I and Anders have applied to Kempe in coop with the chief silviculturist at Holmen and the seed manager of SSP (Lagan, Finnvid). We did not get money. We applied in Bo Rydin but they suggested no spring meeting We applied föreningen but they did not want to initiate something without the full board. We now have an application at Bo Rydin and will send one to föreningen Thus we wait till 2009 with the seeds. One advtage is that the pollen production dropped 2007, but hopefully will raise again 2008. Harvesting 25% of area of Västerhus clonewise The number of seeds will be counted for each clone, thus the female fertility. This can be combined with our measurements of male fertility Follow these harvests through seeds, nursery to the field. Ten halfsib families are chosen for trials 10 field plantations around 20 hectars each with the 10 lots with half sibs and 3 (as comparison) with mixtures We may revise BWB to be applied to half sib forests, when we know the mother

22. Null alleles assumed to be partly present Contamination (%)51,63(+ 3,95) Selfing (%)1,30(+ 0,62) Outcrossing within seed orchad (%) 47,07(+ 4,05) Pollen contamination and self-fertilization, within Västerhus in 9 loci Standard error in parentheses Contamination is high like in most similar studies!! Remains a very big and unsolved problem Selfing is low compared to similar earlier studies, non problematic level

23. Number of seeds Expected (black bars) and observed (grey bars) contributions of each clone as a pollen donor when assuming null alleles in 9 loci Expected value = total no. of seeds with single candidate  No. of ramets representing each clone Total no. of ramets in the seed orchard Observed Expected