1. PRESENTS

2. G Protein Coupled Receptors Tim Hulsen & David Lutje Hulsik produced by

3. G Protein Coupled Receptors seven transmembrane regions hydrophobic/ hydrophilic domains conserved residues and motifs (i.e. NPXXY)

4. Rhodopsin with conserved residues

5. GPCR-G protein coupling receptor gets activated by agonist G protein binds to activated receptor Agonist binding to receptor becomes stronger upon G protein coupling GDP is released G protein takes up GTP GTP uptake triggers release of G protein from receptor Schematic drawing of the flow of events in G protein activation. The process starts when the Arg of the DRY motif moves toward the cytosol. The signal moves `through' helix 5 to the nucleotide binding site. The GDP– GTP exchange leads to a conformational change in the switch region. This leads to dissociation of Gß and it destabilizes the region where the G protein N- and C- terminus come together with the GPCR C-terminus, which leads to G protein dissociation.

6. Studies on GPCRs mutation studies spinlabel NMR cystein scanning protease studies photoaffinity

7. Our Goals to determine whether available data is correct to combine research already done on GPCRs using data from different sources to investigate GPCRs

8. Current Activities working with What-If reading a lot of articles searching for GPCRs on the web website building “programming” deathmatching superpositioning alignment between Rhodopsin and Bacteriorhodopsin

9. GPCR Website http://go.to/gpcr

10. The End Fin Ende Einde

11. Albert VerlindeDavid Lutje HulsikTim Hulsen Director David Lutje Hulsik Producer Tim Hulsen Casting Gert Vriend Set Decoration Wim Janssen Technical assistant Mike Meijnhard ©2000, Tim Hulsen & David Lutje Hulsik