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Rhizobium Activity Manhasset Science Research. Need Bean Yellow Mosaic Virus (Brunt, 1996) Asia leads in soybean production (Westcott, 2008)

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Presentation on theme: "Rhizobium Activity Manhasset Science Research. Need Bean Yellow Mosaic Virus (Brunt, 1996) Asia leads in soybean production (Westcott, 2008)"— Presentation transcript:

1 Rhizobium Activity Manhasset Science Research

2 Need Bean Yellow Mosaic Virus (Brunt, 1996) Asia leads in soybean production (Westcott, 2008)

3 Knowledge Base (Deacon, 2009) Nodule formation on stem causes growth stimulation (Burdass, 2002) Aphids (Homoptera) transmit BYMV (Day, 1996) (Clark, 1998)

4 Knowledge Base González, 2006 Nodule formation processes (Burdass, 2002)

5 Knowledge Base Poole, 2006 Rhizobium is the most important bacterial group in nitrogen fixation (Burdass, 2002)

6 Literature Review “Rhizobium- legume Symbiosis” Department of Molecular & Cell Biology Juan E. González Exopolysaccharides (microorganisms) may play a role in nitrogen fixaton

7 Literature Review “Characterisation of Hardenbergia Mosaic Virus and Development of Microarrays for Detecting Viruses in Plants” Murdoch University Craig Graham Webster Potyvirus can cause malformation of legumes such as these cucumbers

8 Purpose/Hypothesis The purpose of an experiment testing the effects of rhizobium on nodulation processes and aphid extermination is to find which specific type or strand of rhizobium, natural or inoculants, will better form nodules, and decrease aphid activity. H(a) suggests that inoculant rhizobium will better form nodules and exterminate aphids

9 Methods Rhizobium Strain Effects On Nitrogen Fixation and Aphid Extermination Natural rhizobium strains N=10 Oil based inoculant rhizobium strains N=10 5-7 days incubation, sub culturing TY plates, growth in media and nodulation Aphids exposed to nodules Nodules formed in effect of inoculant/ natural strains Percentage of aphids exterminated by inoculant/ natural strains ANOVA, Scheffe post-hoc test: inoculants with natural rhizobium (p<.05)

10 Methods Protocols -Isozation processes -Nodulation testing Taranto, 2003Hollowell, 2009

11 Do-Ability VendorCatalog #Page/WebsiteQuantityDescriptionPriceTotal Carolina Science CatalogNP-15-4720421Rhizobium Inoclum$13.00 $13.00 Carolina Science CatalogNP-70-12963231Lab Incubator$365.00 $365.00 Online Carolina Science Catalog1782001Soybean Seeds$9.00 $9.00 Carolina Science CatalogNP-59-09506161Educational Microscope $225.00 $225.00 Online Carolina Science Catalog8216621Prepared media plates$14.50 $14.50 WebWab Online2441151Homopteran aphid bugs$5.99 $5.99 -Incubators and microscopes will not have to be purchased

12 Citations Brunt, A.A. “Mung Bean Yellow Mosaic Bigeminivirus.” Plant Viruses Online. 1996. Descriptions and Lists from the VIDE Database. 20 Aug 1996 http://image.fs.uidaho.edu/vide/descr503.htm http://image.fs.uidaho.edu/vide/descr503.htm Van Leur, J. “Temperate Pulse Viruses: Bean Yellow Mosaic Virus (BYMV).” DEPARTMENTOF PRIMARY INDUSTRIES. 2002. Symptoms/Economic Importance. 2-7 Feb 2002 http://www.dpi.vic.gov.au/dpi/nreninf.nsf/childdocs/- 22C871BE2A0105794A2568B30004D413- 3C5B474B480825C8CA256BC700824574- 78F1BBD6496F89034A256DEA00274EEC- 494B7E2886B7BE85CA257275000811C1?openhttp://www.dpi.vic.gov.au/dpi/nreninf.nsf/childdocs/- 22C871BE2A0105794A2568B30004D413- 3C5B474B480825C8CA256BC700824574- 78F1BBD6496F89034A256DEA00274EEC- 494B7E2886B7BE85CA257275000811C1?open Burdass, Dariel. “Rhizobium, Root Nodules & Nitrogen Fixation.” Society for general Microbiology. 2002. Microbiology Online. 2002 http://www.microbiologyonline.org.uk/forms/rhizobium.pdf http://www.microbiologyonline.org.uk/forms/rhizobium.pdf Day, Eric. “Aphid.” Virginia Cooperative Extension. 1996. Virginia State University. Aug 1996 http://www.ext.vt.edu/departments/entomology/factsheets/gaphids.html>http://www.ext.vt.edu/departments/entomology/factsheets/gaphids.html González, Juan E. “Faculty and Research.” Department of Molecular & Cell Biology. 2006. Research Interests. 6 Feb 2006 http://www.utdallas.edu/biology/faculty/research/gonzalez.html http://www.utdallas.edu/biology/faculty/research/gonzalez.html Webster, Craig Graham. “Characterisation of Hardenbergia Mosaic Virus and Development of Microarrays for Detecting Viruses in Plants.” Murdoch Research Repository. 2008. Murdoch University. 2008 http://researchrepository.murdoch.edu.au/vital/access/manager/Repository/wmdu:44 3 http://researchrepository.murdoch.edu.au/vital/access/manager/Repository/wmdu:44 3

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