1. Experimentally solving protein structures and protein-protein interactions Lecture 21 Introduction to Bioinformatics 2007 C E N T R F O R I N T E G R A T I V E B I O I N F O R M A T I C S V U E

2. Today’s lecture 1.Experimental techniques for determining protein tertiary structure 2.Protein interaction and docking i.Ribosome example ii.Zdock method 3.Molecular motion simulated by molecular mechanics

3. If you throw up a stone, it is Physics.

4. If you throw up a stone, it is Physics. If it lands on your head, it is Biophysics.

5. If you write a computer program, it is Informatics.

6. If you throw up a stone, it is Physics. If it lands on your head, it is Biophysics. If you write a computer program, it is Informatics. If there is a bug in it, it is Bioinformatics

7. Experimentally solving protein structures Two basic techniques: 1.X-ray crystallography 2.Nuclear Magnetic Resonance (NMR) tchniques

8. 1. X-ray crystallography Purified protein Crystal X-ray Diffraction Electron density 3D structure Biological interpretation Crystallization Phase problem

9. Protein crystals Regular arrays of protein molecules ‘Wet’: 20-80% solvent Few crystal contacts Protein crystals contain active protein Enzyme turnover Ligand binding Example of crystal packing

10. Examples of crystal packing  2 Glycoprotein I ~90% solvent (extremely high!) Acetylcholinesterase ~68% solvent

11. Problematic proteins (no crystallisation) Multiple domains Similarly, floppy ends may hamper crystallization: change construct Membrane proteins Glycoproteins Flexible Lipid bilayer hydrophilic hydrophobic Flexible and heterogeneous!!

12. Experimental set-up Options for wavelength: –monochromatic, polychromatic –variable wavelength Liq.N 2 gas stream X-ray source detector goniometer beam stop

13. Diffraction image Water ring Diffuse scattering (from the fibre loop) reciprocal lattice (this case hexagonal) Beam stop Increasing resolution Direct beam Reflections (h,k,l) with I(h,k,l)

14. The rules for diffraction: Bragg’s law Scattered X-rays reinforce each other only when Bragg’s law holds: Bragg’s law: 2d hkl sin  = n

15. Phase Problem Determining the structure of a molecule in a crystalline sample requires knowing both the amplitude and the phase of the photon wave being diffracted from the sample X-rays which are emitted start out with dispersed phases, and so the phases get lost Unfortunately, phases contribute more to the informational content of a X-ray diffraction pattern than do amplitudes. It is common to refer to phaseless X-ray data as having "lost phases“ Luckily, several ways to recover the lost phases have been developed

16. Building a protein model Find structural elements: –  -helices,  -strands Fit amino-acid sequence

17. Building a protein model Find structural elements: –  -helices,  -strands Fit amino-acid sequence

18. Effects of resolution on electron density Note: map calculated with perfect phases d = 4 Å

19. d = 3 Å Effects of resolution on electron density Note: map calculated with perfect phases

20. d = 2 Å Effects of resolution on electron density Note: map calculated with perfect phases

21. d = 1 Å Effects of resolution on electron density Note: map calculated with perfect phases

22. Refinement process Bad phases  poor electron density map  errors in the protein model Interpretation of the electron density map  improved model  improved phases  improved map  even better model … iterative process of refinement

23. Validation Free R-factor (cross validation) –Number of parameters/ observations Ramachandran plot Chemically likely (WhatCheck) –Hydrophobic inside, hydrophilic outside –Binding sites of ligands, metals, ions –Hydrogen-bonds satisfied –Chemistry in order Final B-factor (temperature) values

24. 2. Nuclear Magnetic Resonance (NMR) 800 MHz NMR spectrometer

25. Nuclear Magnetic Resonance (NMR) Pioneered by Richard R. Ernst, who won a Nobel Prize in chemistry in 1991, FT-NMR works by irradiating the sample, held in a static external magnetic field, with a short square pulse of radio-frequency energy containing all the frequencies in a given range of interest. The polarized magnets of the nuclei begin to spin together, creating a radio frequency (RF) that is observable. Because the signals decays over time, this time-dependent pattern can be converted into a frequency-dependent pattern of nuclear resonances using a mathematical function known as a Fourier transformation, revealing the nuclear magnetic resonance spectrum. The use of pulses of different shapes, frequencies and durations in specifically-designed patterns or pulse sequences allows the spectroscopist to extract many different types of information about the molecule.

26. Nuclear Magnetic Resonance (NMR) Time intervals between pulses allow—among other things— magnetization transfer between nuclei and, therefore, the detection of the kinds of nuclear-nuclear interactions that allowed for the magnetization transfer. Interactions that can be detected are usually classified into two kinds. There are through-bond interactions and through-space interactions. The latter usually being a consequence of the so-called nuclear Overhauser effect (NOE). Experiments of the nuclear-Overhauser variety may establish distances between atoms. These distances are subjected to a technique called Distance Geometry which normally results in an ensemble of possible structures that are all relatively consistent with the observed distance restraints (NOEs). Richard Ernst and Kurt Wüthrich —in addition to many others— developed 2-dimensional and multidimensional FT-NMR into a powerful technique for the determination of the structure of biopolymers such as proteins or even small nucleic acids. This is used in protein nuclear magnetic resonance spectroscopy. Wüthrich shared the 2002 Nobel Prize in Chemistry for this work.

27. Gly Asp Asn Asp Phe Thr Ser Leu Val 2D NOESY spectrum Peptide sequence (N-terminal NH not observed) Arg-Gly-Asp-Val-Asn-Ser-Leu-Phe-Asp-Thr-Gly

28. NMR structure determination: hen lysozyme 129 residues –~1000 heavy atoms –~800 protons NMR data set –1632 distance restraints –110 torsion restraints –60 H-bond restraints 80 structures calculated 30 low energy structures used 0 2000 4000 6000 8000 1 10 4 1.2 10 4 10203040506070 Total energy Structure number

29. Solution Structure Ensemble Disorder in NMR ensemble –lack of data ? –or protein dynamics ?

30. Problems with NMR Protein concentration in sample needs to be high (multimilligram samples) Restricted to smaller sized proteins (although magnets get stronger) Uncertainties in NOEs introduced by internal motions in molecules (preceding slide)

31. X-ray and NMR summary Are experimental techniques to solve protein structures (although they both need a lot of computation) Nowadays typically contain many refinement and energy-minimisation steps to optimise the structure (next topic)

32. X-ray and NMR summary (Cntd.) X-ray diffraction –From crystallised protein sample to electron density map Structure descriptors: resolution, R-factor, B-factor Nuclear magnetic resonance (NMR) –Based on atomic nuclear spin –Produces set of distances between residues (distance restraints) –Distances are used to build protein model using Distance Geometry (a technique to build a protein structure using a set of inter-residue distances)

33. Protein binding and protein-protein interactions Complexity: –Multibody interaction Diversity: –Various interaction types Specificity: –Complementarity in shape and binding properties

34. Protein-protein interactions Many proteins interact through hydrophobic patches Hydrophobic patches often have a hydrophilic rim The patch-rim combination is believed to be important in providing binding specificity hydrophobic very hydrophilic hydrophilic

35. PPI Characteristics Universal –Cell functionality based on protein-protein interactions Cyto-skeleton Ribosome RNA polymerase Numerous –Yeast: ~6.000 proteins at least 3 interactions each  ~18.000 interactions –Human: estimated ~100.000 interactions Network –simplest: homodimer (two) –common: hetero-oligomer (more) –holistic: protein network (all)

36. Interface Area Contact area –usually >1100 Å 2 –each partner >550 Å 2 each partner loses ~800 Å 2 of solvent accessible surface area –~20 amino acids lose ~40 Å 2 –~100-200 J per Å 2 Average buried accessible surface area: –12% for dimers –17% for trimers –21% for tetramers 83-84% of all interfaces are flat Secondary structure: –50%  -helix –20%  -sheet –20% coil –10% mixed Less hydrophobic than core, more hydrophobic than exterior

37. Complexation Reaction A + B  AB –K a = [AB]/[A][B]  association –K d = [A][B]/[AB]  dissociation

38. Experimental Methods for determining PPI 2D (poly-acrylamide) gel electrophoresis  mass spectrometry Liquid chromatography –e.g. gel permeation chromatography Binding study with one immobilized partner –e.g. surface plasmon resonance In vivo by two-hybrid systems or FRET Binding constants by ultra-centrifugation, micro- calorimetry or competition Experiments with labelled ligand –e.g. fluorescence, radioactivity Role of individual amino acids by site directed mutagenesis Structural studies –e.g. NMR or X-ray

39. PPI Network http://www.phy.auckland.ac.nz/staff/prw/biocomplexity/protein_network.htm

40. Binding vs. Localization Obligate oligomers Non-obligate weak transient Non-obligate triggered transient e.g. GTPPO 4 - Non-obligate co-localised e.g. in membrane Non-obligate permanent e.g. antibody-antigen strong weak co-expressed and at same place different places

41. Some terminology Transient interactions: –Associate and dissociate in vivo Weak transient: –dynamic oligomeric equilibrium Strong transient: –require a molecular trigger to shift the equilibrium Obligate PPI: –protomers no stable structures on their own (i.e. they need to interact in complexes) –(functionally obligate)

42. Analysis of 122 Homodimers 70 interfaces single patched 35 have two patches 17 have three or more

43. Interfaces ~30% polar ~70% non-polar

44. Interface Rim is water accessible rim interface

45. Interface composition Composition of interface essentially the same as core But % surface area can be quite different! = different surface/interface areas

46. Some preferences prefer avoid

47. Ribosome structure In the nucleolus, ribosomal RNA is transcribed, processed, and assembled with ribosomal proteins to produce ribosomal subunits At least 40 ribosomes must be made every second in a yeast cell with a 90-min generation time (Tollervey et al. 1991). On average, this represents the nuclear import of 3100 ribosomal proteins every second and the export of 80 ribosomal subunits out of the nucleus every second. Thus, a significant fraction of nuclear trafficking is used in the production of ribosomes. Ribosomes are made of a small and a large subunit Large (1) and small (2) subunit fit together (note this figure mislabels angstroms as nanometers)

48. Ribosome structure The ribosomal subunits of prokaryotes and eukaryotes are quite similar but display some important differences. Prokaryotes have 70S ribosomes, each consisting of a (small) 30S and a (large) 50S subunit, whereas eukaryotes have 80S ribosomes, each consisting of a (small) 40S and a bound (large) 60S subunit. However, the ribosomes found in chloroplasts and mitochondria of eukaryotes are 70S, this being but one of the observations supporting the endosymbiotic theory. "S" means Svedberg units, a measure of the rate of sedimentation of a particle in a centrifuge, where the sedimentation rate is associated with the size of the particle. Note that Svedberg units are not additive. Each subunit consists of one or two very large RNA molecules (known as ribosomal RNA or rRNA) and multiple smaller protein molecules. Crystallographic work has shown that there are no ribosomal proteins close to the reaction site for polypeptide synthesis. This suggests that the protein components of ribosomes act as a scaffold that may enhance the ability of rRNA to synthesise protein rather than directly participating in catalysis. The differences between the prokaryotic and eukaryotic ribosomes are exploited by humans since the 70S ribosomes are vulnerable to some antibiotics that the 80S ribosomes are not. This helps pharmaceutical companies create drugs that can destroy a bacterial infection without harming the animal/human host's cells!

49. 70S structure at 5.5 Å (Noller et al. Science 2001)

50. 70S structure

51. 30S-50S interface Overall buried surface area ~8500 Å 2 < 37.5 Å 2 37.5 Å 2 – 75 Å 2 > 75 Å 2

52. Protein-nucleic acid Interactions

53. Interactions in the Ribosome

54. Calculating interface areas Given a complex AB: 1.Calculate Solvent Accesible Surface Area (SASA) of A, of B, and of AB 1.SASA lost upon complex formation is SASA(A)+SASA(B)-SASA(AB) 3.Interface area of A and of B is (SASA(A)+SASA(B)-SASA(AB))/2

55. Summary protein(-protein) interactions Different binding modes (transient, obligate, also depending on (co)localisation, etc.) Hydrophobic patch/hydrophilic rim conferring binding specificity Interfaces are physico-chemically positioned in between surface and protein core (amino acid composition, etc.) Ribosomes –Small/large subunits, mixture of RNA and protein, different between prokyarotic and eukaryotic cells (exploited by administering antibiotics), ribosomal protein complexes, protein-RNA binding