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Also known as wet chemistry and bench chemistry

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Presentation on theme: "Also known as wet chemistry and bench chemistry"— Presentation transcript:

1 Also known as wet chemistry and bench chemistry
Handling Liquids Robyt and White ; pp Also known as wet chemistry and bench chemistry

2 Water Type of water Contaminants Removed Applications Tap
Coliforms, Solids, Poisons Washing Dishware Tap Distilled (dH2O) Most Minerals (“hard water”) Rinsing Dishes Media Glass Distilled (gdH2O; ddH2O) Minerals from pipe Media, Buffers and reagents De-ionized (dIH2O) Trace volatile organics Bicarbonate Molecular Biology

3 Carboys Hold large quantities 2-20 L
Bulk solutions: TAE Buffer, SSC Buffer Remember to crack the lid!

4 Erlenmeyer Flasks 125 ml -4 liters Melting agar media, agarose
Can be heated and autoclaved Not intended for storage

5 Beakers 100 ml – 2 liters Start solutions, media without agar
Can be heated Not intended for storage

6 Screw-cap Bottles Volumes 100 ml to 1000 ml
Cheap, autoclaveable, reusable Molecular Biology reagents, Liquid Media Do not put on hot plate! Storage

7 Conical Tubes Plastic, sterile, disposable centrifuge tubes
15 ml or 50 ml size Grow bacteria – 5ml media in 50 ml tube Filter-sterilized solutions and media Great for storage (including freezing)

8 Microcentrifuge Tubes
Plastic, sterile, disposable centrifuge tubes 2, 1.5, 0.5, and 0.2 (microamp) formats Most molecular techniques, small reaction volumes Special racks and storage

9 Graduated Cylinders vs Volumetric Flasks

10 Pipetters Air-displacement piston pipettes
Disposable, sterile, plastic tips Volumes 0.5 ml – 1000 ml

11 Downstream


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