1. Also known as wet chemistry and bench chemistry
Handling Liquids
Robyt and White ; pp
Also known as wet chemistry and bench chemistry

2. Water Type of water Contaminants Removed Applications Tap
Coliforms, Solids, Poisons
Washing Dishware
Tap Distilled
(dH2O)
Most Minerals
(“hard water”)
Rinsing Dishes
Media
Glass Distilled
(gdH2O; ddH2O)
Minerals from pipe
Media, Buffers and reagents
De-ionized
(dIH2O)
Trace volatile organics
Bicarbonate
Molecular Biology

3. Carboys Hold large quantities 2-20 L
Bulk solutions: TAE Buffer, SSC Buffer
Remember to crack the lid!

4. Erlenmeyer Flasks 125 ml -4 liters Melting agar media, agarose
Can be heated and autoclaved
Not intended for storage

5. Beakers 100 ml – 2 liters Start solutions, media without agar
Can be heated
Not intended for storage

6. Screw-cap Bottles Volumes 100 ml to 1000 ml
Cheap, autoclaveable, reusable
Molecular Biology reagents, Liquid Media
Do not put on hot plate!
Storage

7. Conical Tubes Plastic, sterile, disposable centrifuge tubes
15 ml or 50 ml size
Grow bacteria – 5ml media in 50 ml tube
Filter-sterilized solutions and media
Great for storage (including freezing)

8. Microcentrifuge Tubes
Plastic, sterile, disposable centrifuge tubes
2, 1.5, 0.5, and 0.2 (microamp) formats
Most molecular techniques, small reaction volumes
Special racks and storage

9. Graduated Cylinders vs Volumetric Flasks

10. Pipetters Air-displacement piston pipettes
Disposable, sterile, plastic tips
Volumes 0.5 ml – 1000 ml

11. Downstream