1. Protein analysis and proteomics II Monday, 30 January 2006 Introduction to Bioinformatics DA McClellan david_mcclellan@byu.edu

2. Proteomics: High throughput protein analysis Proteomics is the study of the entire collection of proteins encoded by a genome “Proteome” refers to all the proteins in a cell and/or all the proteins in an organism Large-scale protein analysis 2D protein gels Yeast two-hybrid Rosetta Stone approach Pathways Page 247

3. Classical biochemical approach Identify an activity Develop a bioassay Perform a biochemical purification Strategies: size, charge, hydrophobicity Purify protein to homogeneity Clone cDNA, express recombinant protein Grow crystals, solve structure Page 247

4. Two-dimensional protein gels First dimension: Isoelectric focusing Separates proteins based on charge Second dimension: SDS-PAGE Sodium Dodecyl Sulfate – PolyAcrylamide Gel Electrophoresis Separates proteins based on size Page 248

5. Fig. 8.19 Page 249

6. Fig. 8.20 Page 252

7. Fig. 8.21 Page 253

8. Fig. 8.21 Page 253

9. Fig. 8.22 Page 254