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Metagenomics Rob Edwards MCS
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The Soudan Mine, Minnesota Red Stuff Oxidized Black Stuff Reduced
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Red and Black Samples Are Different Cloned and 454 sequenced 16S are indistinguishable Black stuff Red Cloned Red
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There are different amounts of substrates in each environment Black Stuff Red Stuff
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There are different amounts of metabolism in each environment
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Nitrification differentiates the samples Edwards (2006)
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The SEED metagenomics server
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Metabolic potential in environments
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Phylogenomics
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Comparing Metagenomes to Genomes (or other metagenomes!)
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Lots of sequences all pyrosequencing
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Metabolic potential in environments
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Sulfur CDA 60.2% CDA 21.7% Respiration Capsule Motility Membrane transport Stress Signaling Phosphorus RNA Mine Saltern Marine Microbialites Coral Fish Animals Freshwater From sequences to environments Dinsdale et al. unpublished
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