1. MCB 730: Southern Blot Lab Southern, Northern & Western blotting Flowchart Detailed Protocol

2. Who is this guy?

3. Sir Edwin Mellor Southern of course (1938- ) Inventor of the Southern blot http://en.wikipedia.org/wiki/Edwin_Southern

4. Characterization: Southern blot hybridization -transfer of DNA from a gel to a membrane (e.g., nitrocellulose, nylon) -developed by Edwin Southern

5. Characterization: Northern blot hybridization -transfer of RNA from a gel to a membrane (e.g., nitrocellulose, nylon) -reveals mRNA size (and approximate protein size), tissue- and organ- specific expression, and kinetic patterns of expression X RNA X x salt X RNA

6. Characterization: Western blotting -transfer of protein from a gel to a membrane (e.g., nitrocellulose, nylon) -requires the use of an electric current to facilitate transfer X Protein X x Buffer; requires electric current X React with Antibody X Enzyme reaction or

7. DNA Microarrays: An Introduction

8. Microarray Result: Much analysis to follow