1. Part 1: Intracellular trafficking Week 1: Transport through the nuclear pore complex Week 2: RNA transport, maturation & localization Week 3: ER translocation and vesicular transport Week 4: Secretion & cell polarity Week 5: Transport into other organelles and organellar homeostasis

2. Part 1: Intracellular trafficking Week 1: Transport through the nuclear pore complex Experimental approaches Signals and pathways The nuclear pore complex Soluble transport factors: import + export The function of the small GTPase Ran Mechanism of translocation

12. I. Signals target proteins into the nucleus a. active transport b. signals are necessary and sufficient Ran-GTP NPC cargo Importin 

14. SUMMARY I. Signals target proteins into and out of the nucleus a. active transport b. signals are necessary and sufficient II.Transport occurs through the nuclear pore complex (NPC) a. NPC has aqueous channel b. no unfolding of cargoes is required c. transport is bi-directional III. Signals are recognized by soluble receptors: importins a. bind specific classes of cargo b. shuttle between cytoplasm and nucleus c. interact with nucleoporins