1. Lecture 4: Statistical Mechanics of Gene Regulation Rob Phillips California Institute of Technology (Beautiful work of David Goodsell)

2. The Single Molecule Census Continued: Gene Expression (Beautiful work of David Goodsell) RNAP+DNA+mRNA Lac Repressor CRP and DNA Products of the Lac Operon

3. All Together Now: The Lac Operon Our goal: mathematize the story told by all of these cartoons. In particular, compute the probability of RNAP binding as a function of concentration of all the other molecular actors.

4. Measure the Concentration of the Gene Product: Fluorescence (Elowitz and Leibler)

5. Repression in the Lac Operon: the Role of Looping (Elowitz et al.)

6. Beta-Galactosidase Activity Ian B. Dodd et al. Genes Dev. 2004; 18: 344-354 (Dove et al.) (Lawrence et al.)

7. Measuring the mRNA Content of a Cell (Yeast microarray data – Brown Group at Stanford)

8. Real-Time PCR www.biorad.com 2a. excitation flters 2b. emission filters 1. halogen tungsten lamp 4. sample plate 3. intensi fier 5. ccd dete ctor 350, 000 pixel s

9. Measuring Promoter Occupancy:ChIP http://www.vincibiochem.it/ChIP-IT.htm

10. Repression in the Lac Operon: The Experimental Backdrop (Muller et al.)