1. Molecular Imaging System

2. General Layout 600 – 900 nm light is used to illuminate fluorescent substances injected into the subject on the image plane.

3. Optigrid Functionality Motors provide 120 o phase shifts and focus –Grid moves on the x-axis 11.11 um to complete a phase change –Improves Clarity –Grid moves 2 mm on the z-axis to focus image –30 line pair/mm on a 10x10 mm square (line pair approx.001 in wide) –Lines spatially modulate the illumination  A portion of the OptiGrid at 200x y xz

4. Image Sharpening Provided by Qioptiq

5. 3D Rendering Provided by Qioptiq

6. Subsystem Breakdown Imaging Unit Mechanica l Lens Mount Grid Mount Software Motor Control Image Capture Image Rendering Optical Lens System Medical Testing Phantom Hand

7. Optical System Review

8. Lens Selection Parameters Focal Length Magnification Box Length = 250 mm Box Height = 200 mm. Lens size

9. Computar C mount Alternative Lenses: Telecentric Lens Zoom Lens Simple Lens

10. OSLO Simulation Simulation by Jeff

13. Rear Projection 45 degrees 90 degrees

14. 440mm Platen to Lens 3mm Lens to Grid

16. Degrees of Motion

18. Motion Solution

19. By Edmund ConstructionAluminum/Stainless Steel Mounting SurfacesPrecision ground Straight Line Accuracy2µm/25mm of travel Position Repeatability±0.00005" ConfigurationsX; solid top or thru hole DrivesMetric Micrometer: 0.01mm graduations/0.5mm coarse Fine Screw: no graduations/64 pitch Load Capacity3 kg normal (horizontal orientation, force against stage) 4.5 kg Ta (vertical orientation, thrust against drive) 1.4 kg Tb (vertical orientation, thrust away from spring) Weight0.078 kg / axis NT56-35 by Edmund

20. Metric Rotary Stages by Edmund Stock No.#55-028 Coarse Rotation360° Total Fine Rotation±5° Total Travel/Knob Rotation70.2 min Horizontal Load Capacity3 kg Weight90g

21. 250mm

22. #PartDescription Quan tityVendor Vendor Part #Price 1Carestream Box 1Carestream 2OptiGrid 1QiOptiq 3Dovetail Optical Rail12" Optical Rail1 Edmund OpticsNT54-40199.75 4OptiGrid Holder 1QiOptiq374352-000 5Three-Screw Ring Mounts95mm Adjustable Len Mount 12.5-76mm1 Edmund OpticsNT36-60549.00 6Small Carrier.5" Dovetail Carrier2 Edmund OpticsNT54-40355.00 7Metric Rotary Stage60mm 360 Rotary Stage2 Edmund OpticsNT55-028231.00 81/4-20 Screws To screw into rails for mounting grid & lens 91/16" Black Polyurethane80A Durometer, 6" x 6"1McMaster8716K15311.74 10Loctite 404 Quick Set (46551)rubber adhesive, 1/3 oz bottle1Ellsworth4655117.69 11Rule printed sticker12" ruled tape1McMaster 12Camera LensComputar C1 Edmund Optics 90.00 13Micro Rotary Stage.75" Rotary Stage1 Edmund OpticsNT56-572260.00 14Aluminum Stock 15Fiber Optic Light Source 1Dolan Jenner 16Grid Transparency 1Output City- 17Linear StageOptional for moving printed transparency 2 - 4 Edmund OpticsNT56-357210.00

23. Software System Capabilities Consist of three separate programs Kodak Molecular Imaging Software Optigrid Motor Control GUI MATLAB –Scripts will be developed as necessary for calibration and depth resolved discrimination Optigrid Calibration –A system specific calibration process will be developed –Process will rely on manual coordination of three separate programs Implement necessary algorithms to achieve depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043

25. Objective: To mathematically investigate and invoke the necessary algorithms to achieve the depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043 Bevilacqua, F., Cuccia, D., Durkin, A., Tromberg, B., “Quantitative analysis and imaging of subsurface heterogeneities using spatially structured illumination,” 2002.

26. Phantom Design Objective: To develop a stable, long-term phantom whose optical properties can be predetermined and reproduced, at selected wavelengths, of human biological tissues of the hand, over a spectral range between 400 and 1,000 nm. Purpose: –Simulate light distributions with a geometry of physical tissue. –Initially testing system designs –Calibration of optical devices. –Create reference measurements of reflective fluorescence. How: –Understand of the key physical and biochemical characteristics of tissue that influence its interaction with light. –Match the absorption coefficient μ a, scattering coefficient μ s, and the anisotropic coefficient g(λ)

27. Design of Phantom 3 components: –Casting material Polyurethane –Absorbing material phthalocyanin dye (Epolight 6084) –Scattering agent Aluminum Oxide (alumina; Al 2 O 3 )

29. Total attenuation coefficient, as a function of concentration, of 0.954-mm-diameter micro-spheres.