2. Many genes have unknown function 30% have unknown function only 9% are experimentally verified The Arabidopsis Genome Initiative, Nature 2000 of the 25,498 predicted Arabidopsis genes:

3. PeGASys Parallel Genome Annotation System Developed by Francis Ouellette at the UBC Bioinformatics Centre Goal: Take Genome annotation to the next level Uses a few automated tools and expert biologists to generate highly annotated genome entries

4. Trend 1. Proliferation of Biological Databases from dozens to hundreds of databases

5. “Over the last few decades, significant advances in computational biology have made it possible to analyze a genome in a fully automated approach. Although this approach is efficient, completely automated analyses can be conservative in that they are careful not to over extend our enthusiastic gene model prediction tools. This approach currently represents our best way to update and present this information in a timely fashion.” -PeGASys Web Page