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Tobin Fricke University of California with Asmahan Abu-Arish, Dr. Michael Elbaum & Dr. Sharon Wolf 3d reconstruction of a helical protein from electron microscopy
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Infection by gene transfer from bacterium to plant Growth factor Enzymes to produce opines Can replace with other genes for transformation Evidence for gene transfer to animal cells Agrobacterium tumefaciens
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electron microscopy Just like optical microscopy, but with electrons instead of photons
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tomography by looking through an object at many angles, determine its internal structure.
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back-projection
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Helical symmetry Translation is equivalent to axial rotation
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micrographs
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particles
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filament picking tool
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automated picking
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Results: T-complex structure hollow helical tube diameter 16 nm pitch 5.2 nm 4.3 VirE2/turn shortening factor ~7 3-domain structure of VirE2 single-strand DNA lies on a shelf at the interior of the tube at radius ~5.5 nm 20 kb ssDNA: ext. length 8. m random coil ~90 nm
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