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Tobin Fricke University of California with Asmahan Abu-Arish, Dr. Michael Elbaum & Dr. Sharon Wolf 3d reconstruction of a helical protein from electron.

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Presentation on theme: "Tobin Fricke University of California with Asmahan Abu-Arish, Dr. Michael Elbaum & Dr. Sharon Wolf 3d reconstruction of a helical protein from electron."— Presentation transcript:

1 Tobin Fricke University of California with Asmahan Abu-Arish, Dr. Michael Elbaum & Dr. Sharon Wolf 3d reconstruction of a helical protein from electron microscopy

2 Infection by gene transfer from bacterium to plant Growth factor Enzymes to produce opines Can replace with other genes for transformation Evidence for gene transfer to animal cells Agrobacterium tumefaciens

3 electron microscopy Just like optical microscopy, but with electrons instead of photons

4 tomography by looking through an object at many angles, determine its internal structure.

5 back-projection

6

7 Helical symmetry Translation is equivalent to axial rotation

8 micrographs

9 particles

10

11 filament picking tool

12 automated picking

13 Results: T-complex structure hollow helical tube diameter 16 nm pitch 5.2 nm 4.3 VirE2/turn shortening factor ~7 3-domain structure of VirE2 single-strand DNA lies on a shelf at the interior of the tube at radius ~5.5 nm 20 kb ssDNA: ext. length 8.  m random coil ~90 nm


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