1. 20040400 XMUGXQ PFS0601 Principles of Fluorescence Spectroscopy Chemistry Department XMU

2. 20040400 XMUGXQ PFS0601 Chapter Six Methods of Fluorometry

3. 20040400 XMUGXQ PFS0601 Methods of fluorometry 6.1 IntroductionIntroduction 6.2 Conventional Fluorometry 6.3 Synchronous Fluorescence ScanSynchronous Fluorescence Scan 6.4 3D fluorescence spectroscopy3D fluorescence spectroscopy 6.5 Derivative fluorescence spectroscopyDerivative fluorescence spectroscopy

4. 20040400 XMUGXQ PFS0601 6.1 Introduction Models Steady-state fluorescence measurement Time-domain fluorescence measurement Time resolved fluorescence Frequency-domain fluorescence measurement I0I0 t I0I0 t I0I0 t Light source

5. 20040400 XMUGXQ PFS0601 6.3 Synchronous Fluorescence Scan SFS Conventional Fluorometry Excitation spectrum Emission spectra 1 2 3 4 F F em Scan ex at a certain em Scan em at a certain ex

6. 20040400 XMUGXQ PFS0601 Synchronous fluorescence scan SFS models Scan ex and em simultaneously Constant-wavelength SFS Constant-energy SFS Variable-angle SFS

7. 20040400 XMUGXQ PFS0601 Constant-wavelength SFS

8. 20040400 XMUGXQ PFS0601 Narrowing spectral band & simplifying emission spectrum

9. 20040400 XMUGXQ PFS0601 Narrowing spectral band & simplifying emission spectrum

10. 20040400 XMUGXQ PFS0601 Multi-component analysis F F em CFS F em SFS

11. 20040400 XMUGXQ PFS0601 Theory Where Ex is the excitation wave function at a given wavelength; Em is the emission wave function at a given wavelength; l is the thickness of the sample; K is the instrumental geometry and related parameters.

12. 20040400 XMUGXQ PFS0601 Choosing the  Generally, the stoke’s shift F

13. 20040400 XMUGXQ PFS0601 Choosing the 

14. 20040400 XMUGXQ PFS0601 Choosing the 

15. 20040400 XMUGXQ PFS0601 Constant-energy SFS PAHs

16. 20040400 XMUGXQ PFS0601 Variable-angle SFS

17. 20040400 XMUGXQ PFS0601 Variable-angle SFS

18. 20040400 XMUGXQ PFS0601 Variable-angle SFS

19. 20040400 XMUGXQ PFS0601 Variable-angle SFS

20. 20040400 XMUGXQ PFS0601 Variable-angle SFS

21. 20040400 XMUGXQ PFS0601 Variable-angle SFS

22. 20040400 XMUGXQ PFS0601 Reference Trends in analytical chemistry, 21(12): 787-798, 2002

23. 20040400 XMUGXQ PFS0601 3 dimensional fluorescence spectroscopy

24. 20040400 XMUGXQ PFS0601 Contour 3D spectra

25. 20040400 XMUGXQ PFS0601 Finger printing

26. 20040400 XMUGXQ PFS0601 Volume integral

27. 20040400 XMUGXQ PFS0601 Derivative fluorescence 芘

28. 20040400 XMUGXQ PFS0601 Derivative fluorescence 峰距法切线法 峰零法

29. 20040400 XMUGXQ PFS0601 application b a

30. 20040400 XMUGXQ PFS0601 application

31. 20040400 XMUGXQ PFS0601 application

32. 20040400 XMUGXQ PFS0601 application Finger printing