1. A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005

2. general background  connection between cellular metabolic pathways and immune system response  surface signals tied to mitochondrial activity and fuel

3. cooperative effort  biology –science driver –cell cultures  growing  conditions –cell counting and physical preparation  engineering –data acquisition –temperature measurements –data reduction –data smoothing

4. background-this project  2 mouse leukemia lines L1210 – not drug resistant L1210/ddp– drug resistant  cultured to 10^6 to 10^8 cells  stressed with bleach –measurable, repeatable differences –different culture conditions, metabolic states

8. data analysis first law of thermodynamics:

10. 00.20.40.60.81 0 0.5 1

11. data smoothing least square curve fit: minimize

13. summary  leverage complementary capacities– specific to people and project  involve students  plunge into a new area

14. other cooperative efforts at UCCS  biomechanics –biology, mechanical engineering  orthopedic mechanics –MERIC, biology, mechanical engineering  feedback controls –MAE, biology  microgravity – MAE  assistive technologies – MATH, biology