Download presentation
Presentation is loading. Please wait.
1
Data Extraction cDNA arrays Affy arrays
2
Stanford microarray database
3
Go into public login select organism and the author
4
If you chose to display data, you can access raw data
6
Display data for each slide and download
7
Or you can chose to data retrieval
8
Click on data analysis to select experiments to download
9
Click on data retrieval, use default selections, select gene name, accession etc.
10
Proceed to data filtering
11
Retrieve data
12
Download preclustering file: unnormlalized data
13
Save unnormalize data as text file
14
Open data file in excel
15
Proceed to gene filtering
16
Click on filter data and download median normalized data
17
You may chose to download raw data for channel 1 and channel 2 to do other normalizations
18
Affymetrix Data: Go to GEO
19
Type in a keyword and select a study
20
Experimental Series Information
21
Pubmed
22
Individual array information
24
Some have.cel files available as suplementary materials
25
Other examples of sample files normalized differently: e.g., MAS5
26
Other examples of sample files normalized differently: e.g., dchip
27
Other examples of sample files normalized differently: e.g., rma
28
You can download the normalized or raw (.cel file) data
29
Normalization Methods for raw data Double channel cDNA (channel intensities) Affymetrix oligo arrays (.cel files)
Similar presentations
© 2024 SlidePlayer.com. Inc.
All rights reserved.