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Presynaptic ReleaseLong Term Potentiation Difference in time scale > 6 orders of magnitude.

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Presentation on theme: "Presynaptic ReleaseLong Term Potentiation Difference in time scale > 6 orders of magnitude."— Presentation transcript:

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2 Presynaptic ReleaseLong Term Potentiation Difference in time scale > 6 orders of magnitude

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4 Early InductionExpression Late

5 LTP Induction Protocols

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7 Blocking NMDA Receptors Blocks LTP

8 Uncaging Ca 2+ Alone Can Induce LTP

9 Long Term Depression

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11 Prot ProtK P P Protein Kinases Catalyze the Phosphorylation of Proteins

12 CaMKII P P

13 Early InductionExpression Late

14 LTP Leads to an Increase in Surface AMPA Receptors

15 LTP Leads to an Increase in Spine Size

16 Early InductionExpression Late

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18 Blocking Protein Synthesis Blocks the Late Phase of LTP

19 LTP TAG Prot

20 Weak Stimulus Followed by a Strong Stimulus Produces LTP

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23 Molecular Makeup of the PSD

24 Scaffolding Molecules

25 PSD Proteins Are In Flux

26 Actin Treadmilling, Capping, Cutting and Cross-Linking

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29 Single-Particle Tracking of AMPA Receptor Diffusion AMPA AB QDOT

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32 Imaging of Isolated PSDs with Immunogold EM

33 EM Tomography of Molecular-Scale Structures in the PSD

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35 Förster Resonance Energy Transfer (FRET)

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37 Super-Resolution Imaging Using FPALM 488 nm507 nm405 nm555 nm572 nm Bleached Dendra2

38 405 nm 555 nm572 nm 488 nm507 nm Super-Resolution Imaging Using FPALM

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