2. Louis Pasteur watches a rabies vaccination

3. Immunity: 2 Branches 1. Non-specific Immunity 2. Specific Acquired Immunity Examples? Specific Has memory

4. Macrophage attacking E. coli RBC monocyte

5. Adaptive Immunity: 2 Branches HumoralCell-mediated

6. T-lymphocyte interacting with a macrophage

7. Overview of Immune System

9. Response to Ag (humoral system)

10. Generic Ab

11. Ribbon diagram of a monoclonal ab

12. Variability of residues in different abs. The 3 hypervariable regions are termed complementarity- determining regions (CDR)

13. Side-view of Angiotensin II bound to F ab. Van der Waals surface contacts

14. A peptide fomr the HIV protease with an anti-protease F ab

17. H-gene expression

18. Recombination signal sequences

25. Animation http://vcell.ndsu.nodak.edu/animations/mrnasplicing/advanced.htm

26. Secretory pathway

28. The Ig superfamily

30. Cells of the Immune System

31. 3.7 x 10 11 cells produced/day!

32. Lymphocytes (derived from lymphoid progenitor) Large, granular

34. (e.g. a macrophage)

35. T-cell Ag-presenting macrophage

36. Subtractive Hybridization Why? 98% of gene expression is the same in B and T cells Rearranged DNA Search for the TCR

39. Gene rearrangements yield a functional TCR.

40. Rearranged genes

42. Ig domains TCR accessory proteins

47. Base pairing between U1 snRNA and the 5’ splice site of an mRNA precursor is necessary but not sufficient for splicing to occur.

48. The U2 snRNP associates with the 5’-end of the intron by base pairing prior to lariat formation. U6 also associates with U2 (not the band). U2 base-pairs with the conserved sequence at the splice branch point and with U6.

53. Animation http://vcell.ndsu.nodak.edu/animations/mrnasplicing/advanced.htm

64. cis-acting sites