1. Using Pseudotorsions to Predict the Consensus Rotamers Kevin Keating Yale University

2. What are Pseudotorsions?

4. Why are they useful? – C3’

5. Why are they useful? – C2’

6. Clustering C3’ endo C2’ endo

7. Clustering C3’ endo C2’ endo

8. Clustering C3’ endo C2’ endo

9. Clustering C3’ endo

10. Comparing with RMSD

12. Structure Building

14. Problem 1: Nucleotide vs. Suite

16. Problem 2: Locating C4’

18. From Pseudotorsions to Rotamers C2’-C3’ puckers Using C4’

19. From Pseudotorsions to Rotamers C2’-C3’ puckers Using C1’

20. From Pseudotorsions to Rotamers C3’-C3’ puckers Using C4’

21. From Pseudotorsions to Rotamers C3’-C3’ puckers Using C1’

22. How to Determine Pucker C2’ endo C3’ endo

23. How to Determine Pucker (Developed by Richardson lab)

24. How to Determine Pucker (Developed by Richardson lab)

25. How to Determine Pucker

27. Putting It (Almost) All Together

29. Distances

31. C3’-C2’ puckers only Distances

32. Putting It All Together

34. Conclusions Is possible to combine pseudotorsions and consensus rotamers Clear applications towards crystallography Other applications