1. T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Rob Learmonth Fluorescence fables for the workshop of FABLS www.usq.edu.au/biophysci

2. T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Rob Learmonth: Interests in fluorescence Broad areas in fluorescence: Education & ResearchEducation & Research spectroscopyspectroscopy development & application of new techniques utilising CLSM & MPLSMdevelopment & application of new techniques utilising CLSM & MPLSM To study: cell membrane structure & dynamicscell membrane structure & dynamics spectroscopic signatures of microbesspectroscopic signatures of microbes –With Ewa Goldys, Hemant Bhatta microbial adaptation processes & mechanismsmicrobial adaptation processes & mechanisms microbial responses to membrane-active agentsmicrobial responses to membrane-active agents brewing & wine fermentationbrewing & wine fermentation fluidity modulation in transgenic frost tolerant wheatfluidity modulation in transgenic frost tolerant wheat www.usq.edu.au/biophysci

3. T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Local developments in instrumentation Centre for Advanced Light Microscopy UQ BrisbaneCentre for Advanced Light Microscopy UQ Brisbane –2001 RIEF UQ/USQ: CLSM & MPLSM –2003 LIEF UQ/USQ: CLSM & MPLSM (2) Macquarie UniversityMacquarie University –2003 LIEF MU/ US/ UNSW/ UTS/ UN/ UW / USQ: Multifunctional CLSM / MPLSM/ FLIM USQUSQ –SLM/Aminco Bowman analog fluorometer –ISS photon-counting fluorometer –BIORAD MRC600 CLSM

4. T h e U n i v e r s i t y o f S o u t h e r n Q u e e n s l a n d Current and emerging challenges in fluorescence For monitoring cell status during bioprocesses development ofFor monitoring cell status during bioprocesses development of –rapid techniques –simple instrumentation Flow cytometry of microorganismsFlow cytometry of microorganisms Development of new specific probesDevelopment of new specific probes Improvement of resolution in optical microscopyImprovement of resolution in optical microscopy Development of live-cell FISH?Development of live-cell FISH?