1. Biology Medicine Engineering Bioreactor Cell Engineering Monitoring &
Control
Cell
Stability &
Selection
Therapeutics
& Diagnostics
Tissue
Engineering
Medicine

2. Cell Analysis Increasing Number of Cell Lines Being Maintained
and Used
Emphasis on Greater Throughput and Precision
More Cellular Assays Using
New Markers and Dyes
Pressure to Reduce Time, Cost and Complexity
Cell Analysis

3. CHONDROPROGENITOR CELLS CULTURED ON CULTISPHER-G
Process outline
PASSAGE PERFORMANCE
Initial Cell Density (cells/ml)
Final Cell Density (cell/ml)
Microcarrier Concentration (mg/ml)
3.3x104
4.95x105
0.6
Passage Length (h)
Passage Population Doubling (-)
Passage Expansion Factor (-)
120
3.91 15
1500 L
100 L
1.1x1013 cells
7 L
106 cells P1 P2 P3 P4 P5 P6
Inoculum (cells)
Microcarriers (mg)
Medium Volume (ml)
Final Cell Number (cells)
1x106 18 30
1.5x107
1.5x107
2.7x102
4.5x102
2.3x108
2.3x108
4.1x103
6.8x103
3.4x109
3.4x109
6.1x104
1x105
5.1x1010
5.1x1010
9.1x105
1.5x106
7.6x1011
7.6x1011
1.4x107
2.3x107
1.1x1013
30 days
EXPANSION PERFORMANCE
Initial Cell Number (cells)
Total Microcarriers (mg)
Total Medium Volume (l)
Final Cell Number (cells)
1x106
1.5x107
2.4x104
1.1x1013
Final Population Doubling (-)
Total Expansion Factor (-)
Total Expansion Time (days)
Total Passage Number (-)
23.44
1.1x107 30 6
1x106
5.4x108
1.6x108
1.1x1014
Initial Cell Number (cells)
Total Area (cm2)
Total Medium Volume (ml)
Final Cell Number (cells)
Final Population Doubling (-)
Total Expansion Factor (-)
Total Expansion Time (days)
Total Passage Number (-)
26.76
1.1x108 30 6
Monolayer

4. CHONDROCYTE EXPANSION
Phase 1
CHONDROCYTE EXPANSION
Biocompatible-biodegradable
scaffold
Phase 2
in vitro FORMATION OF FUNCTIONAL TISSUE EQUIVALENTS
Cell bank for
future uses
Isolated
chondrocytes
Engineered cartilage
Expanded
Cell/scaffold constructs culture in tissue bioreactor
Chondrocyte culture
into monolayer systems
Construct
implantation
Biopsy
removal