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20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.

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Presentation on theme: "20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective."— Presentation transcript:

1 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective

2 UV Field Illumination 100X Objective (1.4 NA) 12.5% intervals INTENSITY=230 123 47

3 20x Vis 10x Vis (2/4/99) 10x UV 20x UV Good Poor Field Illumination

4 5x10x20x40x63x 5x10x20x40x63x Visible Ultraviolet Field Illumination

5 Axial Resolution - Lens Comparison 40x63x100x 610nm 390nm 280nm Axial Distance (  m) FWHM

6 190 nm 410 nm

7 647nm 488nm568nm

8

9 Focal Check Beads

10

11 Comparison of PMT Values

12 Intensity Axial Profile Mirror - VisibleBeads - UV/VisibleFocal Check Beads Spectral Registration Check Methods

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