1. What is apoptosis?? Apoptosis is a morphological term
Apoptosis and programmed cell death frequently
are used interchangeably
Apoptotic death is characterized by:
nuclear condensation
membrane blebbing
phosphotidylserine flipping
DNA degradation into nucleosomal fragments
new gene synthesis in some instances

2. Morphological features of apoptosis
Normal
thymocyte
Apoptotic
Thymocyte
(induced to
die by Dex)
Transmission EM Scanning EM

3. Apoptosis Involves Biochemical As Well As Morphological Changes
Figure 9.18a The Biology of Cancer (© Garland Science 2007)

4. Not in all cases
Table 9.3 The Biology of Cancer (© Garland Science 2007)

5. Figure 9.19 The Biology of Cancer (© Garland Science 2007)

6. Apoptosis plays a key role in regulating lymphocyte cell numbers

7. How do we get rid of corpses??
Why do we get rid of corpses??

8. Find me and eat me signal and phagocyte receptors
Lorenz & Ravichandran
LPC = lipid lysophosphatidylcholine

9. Engulfment of apoptotic cells requires cytoskeletal rearrangment
Lorenz & Ravichandran

10. Induction of Apoptosis – an Overview

11. The Apoptosome
Figure The Biology of Cancer (© Garland Science 2007)

12. Caspases Caspase family tree**
Caspases must be cleaved to be activated
Caspase family tree**
**There are now at least twelve caspases

13. Initiator & Effector Caspases

14. Induction of Apoptosis – an Overview

15. The Bcl-2 Family – Key Modifiers of Apoptosis
Figure 9.25a The Biology of Cancer (© Garland Science 2007)

16. Bcl-2 family members play an important role in
many life and death decisions *

17. Figure 9.27c The Biology of Cancer (© Garland Science 2007)

18. Influence of anti-Apoptotic Gene on Outcome of Oncogene Challenge
Engineering the Mice
Influence of Cooperation on Survival

19. Exrinsic and Intrinsic Death Pathways

20. Death Receptors of the Extrinsic Pathway
Figure 9.31a The Biology of Cancer (© Garland Science 2007)

21. Figure 9.33 The Biology of Cancer (© Garland Science 2007)

22. Figure 9.34 The Biology of Cancer (© Garland Science 2007)