1. Biochemistry Ch6. Exploring Gens 阮雪芬 NTUT Oct 14, 2002

2. Restriction Enzyme

3. Specificities of Some Restriction Endonuclease

4. Gel Electrophoresis

5. Southern Blotting

6. Strategy of the Chain-termination Method for Sequencing DNA

8. Fluorescence Detection

9. A Complete Genome

10. Solid-phase Synthesis of a DNA Chain

11. The First Cycle in PCR

12. Multiple Cycles of PCR (I)

13. Multiple Cycles of PCR (II)

14. Multiple Cycles of PCR (III)

15. DNA and Forensics

16. Joining of DNA Molecules by the Cohesive-end Method

17. Formation of Cohesive DNA

18. Choices Vectors for DNA Cloning in Bacteria pBR322 plasmid Lambda phage M13 phage

19. Genetic Map of The Plasmid pBR322

20. Aternative Infection Modes for Lambda Phage

21. Mutant Lambda Phage as a Cloning Vector

22. EM of M13 Filaments Phage

23. M13 Phage DNA a Cloning and Sequencing Vector

24. Creation of a Genomic Library

25. Screening a Genomic Library for a Specific Gene

26. Probes Generated From a Protein Sequence

27. Diagram of a Yeast Artificial Chromosome (YAC)

28. Chromosome Walking

29. Synthesis of Proinsulin by Bacteria

30. Formation of a cDNA duplex

31. Screening of cDNA

32. Gene Expression Analysis Using Microarrays

33. Monitoring Changes in Yeast Gene Expression

34. Microinjection of DNA

35. Transgenic Mice

36. Rat Growth Hormone- metallothionein Gene Construction

37. Gene Disruption by Homologous Recombination

38. Consequences of Gene Disruption

39. Tumors in Plants

40. Ti Plasmids

41. Electroporation

42. Oligonucleotide-directed Mutagenesis

43. Cassette Mutagenesis

44. The Techniques of Protein Chemistry and Nucleic Acid Chemistry are mutually Reinforcing