1. 微生物應用工業 Ch5. 微生物催化劑 阮雪芬 Nov 19, 2002 NTUT www.ntut.edu.tw/~yukijuan/lectures/Microbio/Nov19.ppt

2. Primary Structure

3. Peptide-bond Formation

4. Amino Acid Sequences Have Direction

5. Components of a Polypeptide Chain Backbone: constant Side chains: variable

6. Cross-links Disulfide bond

7. Amino Acid Sequence of Bovine Insulin

8. Peptide Bond Are Plane Side chain AA1 AA2

10. C-N single bond: 1.49 C=N double bond: 1.27

11. Secondary Structure

12. Structure of Alpha Helix

13. Schematic Views of alpha helices Ball-and-stick Ribbon Cyclindrical

14. Structure of a Beta Strand An antiparallel beta sheet A parallel beta sheet

15. Structure of a Mixed Beta Sheet

16. A Twisted beta Sheet

17. A Protein Rich in Beta Sheets The structure of a fatty acid-binding proteins

18. Structure of a Reverse Turn H bond Reverse turn is also known as the beta turn or hairpin bend

19. Loops on a Proteins Surface Unlike alpha helices and beta strands, loops do not have regular, periodic structure

20. Tertiary Structure

21. Three-dimensional Structure of Myoglobin The first protein to been seen in atomic detail.

22. Distribution of Amino Acids in Myoglobin : Hydrophobic amino acids : Charged amino acids : others

23. Protein Domains The cell-surface protein CD4 consists of four similar domains

24. Quaternary Structure

25. Dimer The Cro Protein of Bacteriophage Lamda

26. Tetramer