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The miracle of N 2 ase: How does it do it? N2 bond energy = 945 kJ/mol!!!! :NN: N2 + 8H+ + 8 e- + 16 MgATP  2 NH3 + H2 + 16 MgADP + 16 Pi.

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Presentation on theme: "The miracle of N 2 ase: How does it do it? N2 bond energy = 945 kJ/mol!!!! :NN: N2 + 8H+ + 8 e- + 16 MgATP  2 NH3 + H2 + 16 MgADP + 16 Pi."— Presentation transcript:

1 The miracle of N 2 ase: How does it do it? N2 bond energy = 945 kJ/mol!!!! :NN: N2 + 8H+ + 8 e- + 16 MgATP  2 NH3 + H2 + 16 MgADP + 16 Pi

2 Root nodules formed by Rhizobium bacteria living symbiotically through nitrogen fixation.

3 N2 + 8H+ + 8 e- + 16 MgATP  2 NH3 + H2 + 16 MgADP + 16 Pi

4 EPR used to monitor S= 3/2 signal under a variety of conditions FeMo protein (FeMoco site)Fe protein

5 Big Mystery: What is this atom???

6 P-cluster Reduced stateOxidized state Strange Sulfur: CN= 6: Creates flexibility for Fe’s?

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9 Nitrogen Binding to the FeMo cofactor of Nitrogenase, Johannes Schimpl, Helena M. Petrilli, and P.E. Blöchl, J. Am. Chem. Soc. 125, 15772-15778 (2003), TU Clausthal Institute of Theoretical Physics Department of Applied Theoretical Physics http://www2.pt.tu-clausthal.de/atp/projects/nitro.html

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