1. Research and Development  Discussion Topics What is it ? What is it ? Who pays, who does it ? Who pays, who does it ? Why do it ? Why do it ? How to do it ? How to do it ?  Case Study Start up company based on university research ( Technology transfer) Start up company based on university research ( Technology transfer)

2. NASA Technology Readiness Levels

4. Percentage of Budget by Agency Source: AAAS GovernmentAgencyPercent DOD55.5 HHS (NIH) 21.1 NASA 8.2 8.2 DOE 6.3 6.3 NSF 3.0 3.0 DHS 0.7 0.7 DOT 0.6 0.6

7. Why Do It?  Innovation drives new technologies /products  Technology Push vs. Product Need Better mouse trap? Better mouse trap?  Answer may depend on TRL Basic vs. applied research Basic vs. applied research

8. How To Do It ?  Specific technical answer depends on field.  Good reference for new methods and tools: R & D magazine (www.rdmag.com) R & D magazine (www.rdmag.com)www.rdmag.com  IP/Patent Search as important as literature review See www.scientific.thomson.com for patent management See www.scientific.thomson.com for patent managementwww.scientific.thomson.com

9. Case Study: Startup Company from UB Research  Buffalo BioBlower Technologies startup based on research project in MAE Dept.  Research on compressive heating of gases led to Air Purification device.  Application to destruction of airborne biological hazards demonstrated.  Funding obtained from DOD for Collective Protection system development.  NYSTAR funding obtain to study transition to healthcare

10. Steps in Buffalo BioBlower (B3) Startup  Seed money from UB for POC experiments  Brief DOD and Congressional Staffers  DOD COLPRO funds further experiments  Receive WNY BDF investment funds  Participate in COLPRO Technology Readiness Evaluation  Congressional appropriation received in 2006 DOD budget for further development

11. Compressive heating of an airstream with a rotary mechanical pump can be used to destroy ANY & ALL airborne biotoxins adapting conventional Roots Blower technology BioBlower concept -uniform, instantaneous volumetric heating (25 o C 300 o C) -one-step mechanism, same pump moves the airstream & kills the pathogens

12. History of MultiRecompressive Heating Extended Operation of Roots Blower Temperature ratio (abs) Pressure ratio ’67-69: MRH concept developed for Calspan Hypersonic Test Facility ’76-’81: LLNL Project ’94: Calspan IR&D on Applications ’96-’02: CUBRC funded studies ’02-present: BioBlower MRH based on compressive heating of gases using Roots-type mechanism

13. University Experimental Protocol injection of constant flow of test spores, Bacillus globigii, aerosol by nebulization into the Bio-Blower sampling of.35% of total air flow out of Bio-Blower impinger traps spores in growth media for culture analysis computer interface controls process parameters 9 x 10 8 CFU/mL

14. Initial Proof of Concept Testing, ‘04 heat treated Bacillus globigii (Bg) spores are continuously injected upstream of blower, then sampled downstream SEM (Scanning Electron Microscopy) of biomaterial which passed through the BioBlower at room temperature, 20 o C at >200 o C temperature 3-4 orders log kill, 99.9% dead in a single pass through BioBlower - experiments performed @ - biotesting supported by

15. How it works ! (we think…) pressure oscillations + temperature kill pathogens -pathogens are in device on ms time scale -achieve kills at much lower temperatures -SEM’s show little or no debris, spores exploded 5 ms

16. DOD interest in BioBlower existing systems use passive filtration for biological agent protection, a logistic nightmare! Over 400 suppliers of HEPA filter systems to the military BioBlower does NOT use : filters/UV lights/toxic chemicals BioBlower offers improvements in effectiveness, logistics, operations and power consumption. Kill pathogens on site! No replaceables needed

17. S&T Funded BioBlower Testing, ‘05  Objectives Develop Collection System to Obtain More Quantitative Measure of Kill Effectiveness Develop Collection System to Obtain More Quantitative Measure of Kill Effectiveness Obtain data at 240 o, 270 o and 300 o C Obtain data at 240 o, 270 o and 300 o C Assess effects of organic bioload and dry spores Assess effects of organic bioload and dry spores  Further objective Demonstrate blower operation at 300 o C and 50 CFM Demonstrate blower operation at 300 o C and 50 CFM

18. Observe 6.5 log kill, in a single pass  Filter Collection System Developed to Significantly Increase Spore Count Glass fiber filters difficult to use in flow environment Glass fiber filters difficult to use in flow environment Experiments run at 240 o, 270 o and 300 o C Experiments run at 240 o, 270 o and 300 o C Successful data collection at 240 o C Successful data collection at 240 o C glass fiber filter housing Bst spores 25 o C, 1,000,000 spores collected 240 o C, 1 spore collected 99.9999% killed in single pass

19. Current Status of Company  University files patent application based on POC results  B3 formed and obtained exclusive IP rights from university  First generation prototype tested in TRE, device performed well  DOD 2006 funding being used to build and test next generation prototype  Advanced development/ technology demonstration funding being sought for 2008

20. Key Steps in Start-Up Process  Proof of Concept  IP Protection Patent application Patent application Licensing agreement Licensing agreement  Securing Investment BDF investment ($200K for 20% share) BDF investment ($200K for 20% share) DOD S&T funding ($200K + $50K UB match) DOD S&T funding ($200K + $50K UB match) Congressional DOD appropriation ($1.5M) Congressional DOD appropriation ($1.5M)