1. G.007 Hardware open-CAD 30 msec move/settle 42 kbp/sec 10-60 Gbp/run
Xenon Light
EM-CCD Camera
30 msec move/settle
42 kbp/sec
10-60 Gbp/run
$124,000
Fluidics
Autofocus
Objective
Stage
3U Imaging
2U Sequencing

2. Hardware: Polonator G.007 DIY PersonalGenome.org
Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics
Shendure, Porreca et al. 2005
Danaher, Hamamatsu, Leica
$124K with computer & 1 yr service
Contact:
Rich Terry et al. 2007
DIY
PersonalGenome.org

3. 36 Flowcells G.007 60M beads per flowcell Filling with beads
PersonalGenome.org

4. Higher density & accuracy
Gel array, 12M beads
Shendure, Porreca et al. 2005
Glass array, 60M beads
Kim, Porreca et al. 2007
1.6M reads
12M reads
(M. Umbarger)