1. Introductory Microbiology Dr. Heather Townsend Summer 2009

2. Microbiology The study of of organisms (microorganisms or microbes) too small to be seen without magnification This includes: 1.Bacteria 2.Viruses 3.Fungi 4.Protozoa 5.Helminths (worms) 6.Algae

3. The Microbes 1. Bacteria –Single-celled organisms –Various shapes Spherical Rod Spiral shapes –Cellular –Lack membrane- enclosed cellular structures –Widely distributed in nature Klebsiella pneumoniae, bacteria that causes pneumonia in humans

4. The Microbes 2. Viruses –Acellular –Composed of nucleic acid and a few proteins –Replicate themselves to display other properties of living organisms when they invade living cells Bacteriophages observed at 35,500X

5. The Microbes 3. Fungi –Yeasts and molds Single-celled, microscopic –Mushrooms Multicellular, macroscopic –Cell nucleus and other cellular structures –Absorb nutrients from their environment –Widely distributed in water and soil –Act as decomposers of dead organisms Philobolus crystallinus, fruiting bodies of this fungus

6. The Microbes 4. Protozoa –Single-celled, microscopic organisms –Have at least one nucleus and many cellular structures –Obtain food by engulfing or ingesting smaller organisms –Most can move –Found in many different environments Amoeba

7. The Microbes 5. Misc. –Large, multicellular –Worms Helminths –Arthropods Insects Tapeworm Head

8. The Microbes 6. Algae –Single-celled microscopic organisms –Have a nucleus and many membrane- enclosed cellular structures –Photosynthesize their own food –Widely distributed in fresh and salt water –Important source of food for other organisms Micrasterias, a green algae living in fresh water.

9. Branches of Study Within Microbiology Immunology: –studies immune chemicals and cells that are produced in response to infection Public health microbiology & epidemiology: –aim to monitor and control the spread of diseases (CDC) Food, dairy and aquatic microbiology: –examine the ecological and practical roles of microbes in food and water Biotechnology: –ranges from bread making to gene therapy Genetic engineering & recombinant DNA technology: –altering the genetic makeup of organisms

10. Microbes Are Involved In: nutrient production & energy flow –i.e., photosynthesis decomposition and nutrient recycling production of foods, drugs & vaccines bioremediation causing disease

12. Impact of Pathogens Pathogens –Diseases-causing agents Nearly 2,000 different microbes cause diseases in the human body 10 B infections/year worldwide 13 M deaths from infections/year worldwide killing about 1/3 of the U.S. population each year

13. Impact of Pathogens Emerging diseases –Becoming more prominent over the years –Zoonosis SARS Reemerging –Older diseases increasing in occurrence TB Malaria Hepatitis Noninfectious diseases

14. Characteristics of Microbes Prokaryotic cells –Smaller –Lack special structures such as a nucleus and organelles –All prokaryotic cells are microorganisms Only some microorganisms are eukaryotic

15. Lifestyles of Microorganisms Free existence Close associations –Parasites –hosts

16. Antonie van Leeuwenhoek First to observe living microbes His single-lens magnified up to 300X (1632-1723)

17. Early Medical Microbiology Louis Pasteur: –Worked in the wine industry Had knowledge about yeast producing alcohol –Swan-neck flasks –Tipping the flask would allow the microbes to enter the infusion Cause them to become cloudy Main experiment that helped disprove spontaneous generation

18. Pasteur discovered that some yeasts made good tasting wine Mixtures of microbes competed with yeast and made wine taste oily or sour Developed Pasteurization to deal with this problem –Heated the wine to 56°C without oxygen present for 30 minutes Developed a rabies vaccine Early Medical Microbiology

19. Robert Koch (~120 years ago, German) –Linked a microscopic organism with a specific disease (anthrax) –Developed method to grow bacteria in pure cultures (cultures containing only one kind of organism) Used solidified gelatin from potato slices mixed with agar Creates a firm surface that microbes could grow on

20. Koch’s Postulates 1.The specific causative agent must be found in every case of the disease 2.The disease organism must be isolated in pure culture 3.Inoculation of a sample of the culture into a healthy, susceptible animal must produce the same disease 4.The disease must be covered from the inoculated animal

21. Early Medical Microbiology Oliver Wendell (American physician) –observed mothers who gave birth at home experienced fewer infections than those that gave birth in a hospital Ignaz Semmelweis (Hungarian doctor) –showed that women became infected with puerperal fever during delivery by doctors coming directly from the autopsy room

22. Early Medical Microbiology Joseph Lister (English surgeon) –Introduced aseptic techniques Aimed at reducing microbes in a medial setting and preventing wound infections –Improved sanitation Promotes use of carbolic acid on bandages and medical instruments

23. Spontaneous Generation Theory Germ theory of disease: –“Microorganisms can invade other organisms and cause disease” –Many diseases are caused by the growth of microbes in the body and not by sins, bad character, or poverty, etc. Spontaneous generation –“Living things arise from nonliving things” –Belief that some forms of life could arise from vital forces present in nonliving or decomposing matter Maggots found in rotting meat arose from a nonliving factor Microorganisms found in broth that made it cloudy appeared from a nonliving factor

24. What to expect…….. Different microorganisms How to detect microorganisms Common disease caused by microorganisms How to control the spread of microorganisms Immune system