1. General Pathology: Introduction to the Course
Lorne Holland, M.D.

2. General Information COURSE DIRECTOR: Lorne Holland, M.D.
Leprino Office Building, Main Laboratory- Rm 280
Office: (720)
ADMINISTRATIVE COORDINATOR:
Jaime Kean
Building A01 Room L
Phone (303)

3. Map
Leprino
Office
Building
Parking
Garage

4. Exams
Sample questions will periodically be posted on Blackboard with answers following shortly
Before each exam there will be a Q&A session
This is an opportunity for you to ask questions about the material
This is not meant to be a formal review of all lecture material
For best responses, please send me your question(s) a day or two in advance so I know what to expect

5. Exams Multiple-choice, single best answer 2-3 questions per lecture
Mid-October and December 17th
2nd exam is cumulative with approximately 80% new and 20% old
Exams will be timed with 1.5 minutes allotted for each question

6. Exams How much does a normal human head weigh?
More than twice the average orange
Between 4.4 and 5.3 kilograms
Hair is typically 0.2% to 1.1% of head weight
It would weigh only 1/6th as much on the moon

7. Exams Review session immediately after Answers will be projected
Your chance to ask questions
Only opportunity to review test answers

8. Exams
Grading
Tests are sent to the computer center for grading where they calculate statistics for each question
A few questions will have “bad” numbers and will be thrown out
Too hard or too easy
Random answer pattern

9. Other Tests Independent study questions Periodic, unannounced quizzes
Posted on Blackboard and due Sept 28th
May collaborate, but each student must submit their own answers
Remember, this material is fair game for both exams
Periodic, unannounced quizzes
Multiple-choice, single best answer and/or short answer
You can use class notes and textbook
Students not present when the quizzes are handed out will receive a zero
Lowest quiz score will be dropped

10. Objectives Each lecture will have a set of objectives
This is the bare minimum you should be getting out of each lecture
If you can not perform the objectives then it is unlikely you will pass
If you only know material specifically addressed by the objectives, you will probably pass, but only barely (70-80%)

11. Odds and Ends
Lecture handouts should be available on Blackboard 48 hrs before lecture
Exam dates will be firm, but lectures may occasionally move around a bit. You should have at least 24 hrs warning.
Please address questions directly to the lecturer. If you do not receive a response within 48 hrs then let me know
Evaluations after each test. Please complete them so I know what is working and what is not

12. “laboratory medicine”
Scope of Pathology
Pathology
Experimental
Anatomic
Clinical
“laboratory medicine”

13. Scope of Pathology Pathology Experimental Anatomic Clinical
Pathogenesis
Etiology
Epidemiology

14. Scope of Pathology Pathology Experimental Anatomic Clinical Surgical
Gross / Micro
Autopsy
Gross / Micro
Cytology
Microscopic
Hematopath
Microscopic

15. Scope of Pathology Pathology Experimental Anatomic Clinical Chemistry
Urinalysis
Hematology
Serology
Microbiology
Virology
Molecular
T.D.M.
Toxicology
Coagulation
Transf. Med
Point of Care
Flow Cytometry

16. Quick Guide to Microscopy
Unless otherwise stated, pictures in books and lecture and hemotoxylin and eosin (H&E)
Proteins and starch stain pink
Nucleic acids (DNA, RNA) stain blue
Air, water, sugar and lipids do not stain at all
Naturally colored materials, black, brown, green

17. Quick Guide to Microscopy

18. Quick Guide to Microscopy

19. Other Colors Golden (yellow/brown/green) Brown Black Bile
Hemosiderin, lipofuschin (iron)
Black
Carbon
Melanin

20. Special Stains PAS  starch/sugar Prussian Blue  iron
Oil Red O  lipids

21. Immunohistochemistry
Produce antibodies to specific molecules, usually proteins
Attach a marker of some kind to the antibody
Wash a tissue section with the labeled antibody

22. Keratin-
Carcinoma, not lymphoma
Estrogen receptor-
Breast cancer
CD15/30-
Hodgkin Lymphoma
Alphafetoprotein-
Testicular Cancer

23. Questions?