1. Lecture 33 11/28/05 1 seminar left  Urban sprawl  Today at 4 No lab report will be due on Tuesday, November 29  For this week only, you can skip all the data analysis. Spectroscopy  Some interesting applications

2. Scatchard Plot To get equilibrium constant P + X ⇄ PX Plot ΔA/[X] vs. ΔA Two cases  K small  K large

3. Job’s Plot Method of continuous variation Way to determine the formula of a complex ion P + X  ?????

4. Plot: Absorbance vs. mole fraction X-axis

7. Enzyme-Linked Immunosorbent Assay (ELISA)

8. Time-resolved Fluorescence Immunoassay

9. Microtox/Deltatox

10. Chromatography

11. Extraction Partition coefficient:

12. After 1 extraction After n extractions

13. Solute A has a partition coefficient of 3 between toluene and water. Which is a more efficient extraction of a 100- mL 0.010 M aqueous solution:  One extraction with 500-mL toluene  Five extractions each with100-mL toluene

14. Distribution coefficient