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DNA Nucleosides Deoxyadenosine (A) Deoxyguanosine (G) Deoxycytidine (C) Deoxythymidine (T)

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Presentation on theme: "DNA Nucleosides Deoxyadenosine (A) Deoxyguanosine (G) Deoxycytidine (C) Deoxythymidine (T)"— Presentation transcript:

1

2 DNA Nucleosides Deoxyadenosine (A) Deoxyguanosine (G) Deoxycytidine (C) Deoxythymidine (T)

3 Synthetic Objective G - A - T - T - A - C - A C -phosphate-A -phosphate-T -phosphate- A -phosphate-G- phosphate-A The problem is like the problem of protein synthesis Protection ! The solution?

4 Phosphoramidites A G C T

5 DMT protection A G C T

6 DMT deprotection

7 Protect amines

8 Phosphoramidite converted to Phosphite A C Silica surface tetrazole - diisopropylamine

9 Remove the dimethoxyltrityl group with acid CHCl 2 COOH

10 Next step tetrazole

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