1. Stoichiometry and Kinetics of Cell Growth and Product Formation

3. BASIC TERMINOLOGY
Anchorage-dependent Attachment to a suitable substrate is required for multiplication
Anchorage-independent Multiplication occurs in suspension (in either liquid or semi-solid medium)
Anchorage-preferred Attachment to a suitable substrate is not necessary but preferred for multiplication
Basal medium A mixture defined nutrient dissolved in a buffered physiological saline solution
Chemically defined medium A medium prepared from reagent grade water and highly purified supplements – Trace impurities may be present but at levels too low to influence cellular growth responses
Conditioning The modification of the cellular environment by product released by cells into the medium
Growth factor A non-nutrient substance that controls proliferation in a regulative manner through interaction with specific cellular receptors
Growth medium A mixture of nutrients that promotes cell multiplication
Hormone Chemical substances that are transported through the body and affect target
cells at locations remote from cells that produce them
Maintenance medium A mixture of nutrients and supplements that maintains the structural and metabolic integrity of the cells (cell viability) but does not promote cell division.
Nutrient A low molecular weight substance that enters cells and is utilized weather as substrate for biosynthesis or metabolism or else as a catalyst in those processes
Peptone Acid or enzyme hydrolysate of fresh animal carcass, muscle or offal
Supplement Any non-nutrient substance needed for cell growth (usually high molecular weight)
Serum The liquid remaining after allowing freshly collected blood to clot
Serum-free medium A basal medium that lacks whole or processed serum supplementation. Serum-free medium can and usually does contain one or more protein (or non-protein) component extracted and partially purified from serum or tissue

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