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What is SV40 T-antigen? What does it do? Presented by 李柏毅 Genome Engineering Laboratory, Institute of Bioinformatics, National Yang-Ming University 2003/12/12.

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Presentation on theme: "What is SV40 T-antigen? What does it do? Presented by 李柏毅 Genome Engineering Laboratory, Institute of Bioinformatics, National Yang-Ming University 2003/12/12."— Presentation transcript:

1 What is SV40 T-antigen? What does it do? Presented by 李柏毅 Genome Engineering Laboratory, Institute of Bioinformatics, National Yang-Ming University 2003/12/12

2 2 Simian Virus 40 Hosts: monkeys and mice DNA virus with 5,227 base pairs Origin Early mRNAs Late mRNAs 5’ SV40

3 3 SV40 T-antigen AUG UUA Early pre-mRNA Large T mRNA Small T mRNA AAA n Large T 94kd protein Small T 17kd protein

4 4 Large T-antigen Delete 1-126 a.a. Delete 136-C terminus Addition 127-132 a.a to β-galactosidase and pyruvate kinase Delete 127-132 a.a. Immunofluorescence microscopy Daniel Kalderon, Bruce L. Roberts, Alan E. Smith, A Short Amino Acid Sequence Able to Specify Nuclear Location, Cell 39:499-509, 1984

5 5 Tumor Virus Human cells are semipermissive cell

6 6 Tumor Virus SV40 Large T Ad5 E1A 13S Ad5 E1A 12S Ad5 E1B 55K Rb-bindingP53-binding Release transcription factor for Rb Protein binding sites

7 7 THANKS!

8 8 Hahn WC, et al,. Creation of human tumor cells with defined genetic elements. Nature 400, 464–468 (1999)

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