1. Recombination Early Experiments: 1947 Alfred Hershey and Max Delbrück 1961 Matthew Meselson and Jean Weigle *Homologous Genetic Recombination *Site Specific Recombination *Transposition

2. Homologous Recombination *Two broad categories (pathways):

3. Homologous Recombination

5. RecBCD Pathway http://www.esrf.eu/news/spotlight/spotlight13dnarepair/spotlight_dna_repair_fig1s

6. RecBCD Pathway RecA filament

7. RecA at work

8. RuvAB facilitate branch migration

10. Gene Targeting in Embryonic Stem Cells www.nobelprize.org

11. Gene Targeting in Embryonic Stem Cells www.nobelprize.org