1. (1)Start lasers, then find sample using VIS

2. (2)Set configuration using LSM

3. (3)Set scan resolution, speed & repetition in Mode Initial pixal number, scan speed, averaging, zoom and pinhole size are set in stored configurations. These should be optimised after preliminary images.

4. (4)Set pinhole, excitation & detection for each Channel Single track Multi trackUse Find to get initial image, Split and Palette to adust

5. (5)Control Zoom and Crop in Mode and Select

6. (6)Set Z stack with first/last & optical slice

7. (7)Display 3D using projection

8. (8)Open database for image retrieval

9. (9)Time Series and Edit Bleaching

10. (10)Using Lambda stacks, Save to Spectra DB for Linier Unmixing