1. Bottom line on databanks

2. Relational vs flat-file

3. data tables SQL SQL can be used to connect/join and search tables Example: GI numbers -> sequence and GI numbers to taxonomic information

4. data warehouse

5. Inteins Protein Introns Found in Bacteria, Phage, Eukaryotes and Archaea Many contain homing endonucleases DNA RNA Protein Extein Intein + Host Protein Splicing and Homing Endo- nuclease Domains

6. Small and large Inteins

8. Large Intein with DNA

9. Retained as Functional Endonuclease Homing Cycle Degeneration Acquisition of New Function Precise Loss Intein/Intron without or with Dysfunctional Homing Endonuclease Intein/Intron with Functional Homing Endonuclease Intein/Intron with HE from another Gene, Species, Population, or Subpopulation. Transfer of Homing Endonuclease and Invasion of Local Population Empty Target Site